Oral feeding of Lactobacillus bulgaricus N45.10 inhibits the lung inflammation and airway remodeling in murine allergic asthma: Relevance to the Th1/Th2 cytokines and STAT6/T-bet.
- 2019-07
- Cellular Immunology 341
- E. Anatriello
- M. Cunha
- J. Nogueira
- J. Carvalho
- A. K. Sá
- M. Miranda
- H. Castro-Faria-Neto
- A. Keller
- F. Aimbire
- PubMed: 31178059
- DOI: 10.1016/J.CELLIMM.2019.103928
Abstract
Asthma is a chronic disease with impacts on public health. It affects the airways causing pulmonary inflammation mediated by CD4 T cells type Th2, eosinophilia, mucus hypersecretion, and elevated IgE. The unbalance between cytokines and transcription factors is an important feature in asthma. Probiotics has gaining highlight as a therapy for chronic diseases. Thus, we investigate the Lactobacillus bulgaricus (Lb) effect in murine allergic asthma. BALB/c-mice were sensitized to ovalbumin (OA) on days 0 and 7 and were challenged from day 14-28 with OA. Mice received Lb seven days prior to sensitization and it was kept until day 28. The Lb attenuated the eosinophils infiltration, mucus and collagen secretion, IgE production, pro-inflammatory cytokines, TLR4 expression, GATA3, STAT6 and RORγt in lung. Otherwise, Lb increased the anti-inflammatory cytokines, the T-bet and foxp3. Finally, Lb attenuated the allergic asthma-induced inflammation and airway remodeling by interfering on Th1/Th2 cytokines and STAT6/T-bet transcription factors.
Keywords: Allergic asthma; Cytokines; Mice; Probiotic; Toll-like receptor; Transcriptions factor.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus bulgaricus Lb-87 | Enhanced T-bet Expression | Beneficial | Moderate |
| Lactobacillus bulgaricus Lb-87 | Increased Anti-Inflammatory Cytokine Levels | Beneficial | Moderate |
| Lactobacillus bulgaricus Lb-87 | Increased Foxp3 Expression | Beneficial | Moderate |
| Lactobacillus bulgaricus Lb-87 | Reduced Airway Inflammation Score | Beneficial | Moderate |
| Lactobacillus bulgaricus Lb-87 | Reduced Airway Remodeling | Beneficial | Moderate |
| Lactobacillus bulgaricus Lb-87 | Reduced Collagen Secretion | Beneficial | Moderate |
| Lactobacillus bulgaricus Lb-87 | Reduced Eosinophil Infiltration | Beneficial | Moderate |
| Lactobacillus bulgaricus Lb-87 | Reduced GATA3 Expression | Beneficial | Moderate |
| Lactobacillus bulgaricus Lb-87 | Reduced Mucus Secretion | Beneficial | Moderate |
| Lactobacillus bulgaricus Lb-87 | Reduced Pro-inflammatory Cytokine Levels | Beneficial | Moderate |
| Lactobacillus bulgaricus Lb-87 | Reduced RORγt Expression | Beneficial | Moderate |
| Lactobacillus bulgaricus Lb-87 | Reduced STAT6 Gene Expression | Beneficial | Moderate |
| Lactobacillus bulgaricus Lb-87 | Reduced Serum Immunoglobulin E Levels | Beneficial | Moderate |
| Lactobacillus bulgaricus Lb-87 | Reduced TLR4 Expression | Beneficial | Moderate |