Oral intake of Lactobacillus plantarum L‐14 extract alleviates TLR2‐ and AMPK‐mediated obesity‐associated disorders in high‐fat‐diet‐induced obese C57BL/6J mice
- 2021-04-08
- Cell Proliferation 54(6)
- Jaehoon Lee
- Sangkyu Park
- Naeun Oh
- Jaehyun Park
- Mijin Kwon
- Jeongmin Seo
- S. Roh
- PubMed: 33830560
- DOI: 10.1111/cpr.13039
Abstract
Objectives: Whether periodic oral intake of postbiotics positively affects weight regulation and prevents obesity-associated diseases in vivo is unclear. This study evaluated the action mechanism of Lactobacillus plantarum L-14 (KTCT13497BP) extract and the effects of its periodic oral intake in a high-fat-diet (HFD) mouse model.
Materials and methods: Mouse pre-adipocyte 3T3-L1 cells and human bone marrow mesenchymal stem cells (hBM-MSC) were treated with L-14 extract every 2 days during adipogenic differentiation, and the mechanism underlying anti-adipogenic effects was analysed at cellular and molecular levels. L-14 extract was orally administrated to HFD-feeding C57BL/6J mice every 2 days for 7 weeks. White adipose tissue was collected and weighed, and liver and blood serum were analysed. The anti-adipogenic mechanism of exopolysaccharide (EPS) isolated from L-14 extract was also analysed using Toll-like receptor 2 (TLR2) inhibitor C29.
Results: L-14 extract inhibited 3T3-L1 and hBM-MSC differentiation into mature adipocytes by upregulating AMPK signalling pathway in the early stage of adipogenic differentiation. The weight of the HFD + L-14 group (31.51 ± 1.96 g) was significantly different from that of the HFD group (35.14 ± 3.18 g). L-14 extract also significantly decreased the serum triacylglycerol/high-density lipoprotein cholesterol ratio (an insulin resistance marker) and steatohepatitis. In addition, EPS activated the AMPK signalling pathway by interacting with TLR2, consequently inhibiting adipogenesis.
Conclusions: EPS from L-14 extract inhibits adipogenesis via TLR2 and AMPK signalling pathways, and oral intake of L-14 extract improves obesity and obesity-associated diseases in vivo. Therefore, EPS can be used to prevent and treat obesity and metabolic disorders.
Keywords: Lactobacillus plantarum; AMPK signalling pathway; Toll-like receptor 2; exopolysaccharide; metabolic disorders; obesity.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus plantarum 14D | Improved Insulin Sensitivity | Beneficial | Moderate |
| Lactobacillus plantarum 14D | Reduced Steatohepatitis | Beneficial | Moderate |
| Lactobacillus plantarum 14D | Reduced Weight Gain | Beneficial | Moderate |
| Lactobacillus plantarum L-137 | Improved Insulin Sensitivity | Beneficial | Moderate |
| Lactobacillus plantarum L-137 | Reduced Adipogenesis | Beneficial | Moderate |
| Lactobacillus plantarum L-137 | Reduced Body Weight | Beneficial | Moderate |
| Lactobacillus plantarum L-137 | Reduced Hepatic Steatosis | Beneficial | Moderate |
| Lactobacillus plantarum N13 | Improved Insulin Sensitivity | Beneficial | Moderate |
| Lactobacillus plantarum N13 | Reduced Adipogenesis | Beneficial | Moderate |
| Lactobacillus plantarum N13 | Reduced Body Weight | Beneficial | Moderate |
| Lactobacillus plantarum N13 | Reduced Liver Fat | Beneficial | Moderate |