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Abstract

Objectives: Whether periodic oral intake of postbiotics positively affects weight regulation and prevents obesity-associated diseases in vivo is unclear. This study evaluated the action mechanism of Lactobacillus plantarum L-14 (KTCT13497BP) extract and the effects of its periodic oral intake in a high-fat-diet (HFD) mouse model.

Materials and methods: Mouse pre-adipocyte 3T3-L1 cells and human bone marrow mesenchymal stem cells (hBM-MSC) were treated with L-14 extract every 2 days during adipogenic differentiation, and the mechanism underlying anti-adipogenic effects was analysed at cellular and molecular levels. L-14 extract was orally administrated to HFD-feeding C57BL/6J mice every 2 days for 7 weeks. White adipose tissue was collected and weighed, and liver and blood serum were analysed. The anti-adipogenic mechanism of exopolysaccharide (EPS) isolated from L-14 extract was also analysed using Toll-like receptor 2 (TLR2) inhibitor C29.

Results: L-14 extract inhibited 3T3-L1 and hBM-MSC differentiation into mature adipocytes by upregulating AMPK signalling pathway in the early stage of adipogenic differentiation. The weight of the HFD + L-14 group (31.51 ± 1.96 g) was significantly different from that of the HFD group (35.14 ± 3.18 g). L-14 extract also significantly decreased the serum triacylglycerol/high-density lipoprotein cholesterol ratio (an insulin resistance marker) and steatohepatitis. In addition, EPS activated the AMPK signalling pathway by interacting with TLR2, consequently inhibiting adipogenesis.

Conclusions: EPS from L-14 extract inhibits adipogenesis via TLR2 and AMPK signalling pathways, and oral intake of L-14 extract improves obesity and obesity-associated diseases in vivo. Therefore, EPS can be used to prevent and treat obesity and metabolic disorders.

Keywords: Lactobacillus plantarum; AMPK signalling pathway; Toll-like receptor 2; exopolysaccharide; metabolic disorders; obesity.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus plantarum 14DImproved Insulin SensitivityBeneficial
Moderate
Lactobacillus plantarum 14DReduced SteatohepatitisBeneficial
Moderate
Lactobacillus plantarum 14DReduced Weight GainBeneficial
Moderate
Lactobacillus plantarum L-137Improved Insulin SensitivityBeneficial
Moderate
Lactobacillus plantarum L-137Reduced AdipogenesisBeneficial
Moderate
Lactobacillus plantarum L-137Reduced Body WeightBeneficial
Moderate
Lactobacillus plantarum L-137Reduced Hepatic SteatosisBeneficial
Moderate
Lactobacillus plantarum N13Improved Insulin SensitivityBeneficial
Moderate
Lactobacillus plantarum N13Reduced AdipogenesisBeneficial
Moderate
Lactobacillus plantarum N13Reduced Body WeightBeneficial
Moderate
Lactobacillus plantarum N13Reduced Liver FatBeneficial
Moderate
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