Oral probiotic activities and biosafety of Lactobacillus gasseri HHuMIN D
- 2021-03-23
- Microbial Cell Factories 20(1)
- Soyon Mann
- Myeong-Soo Park
- Tony V Johnston
- G. Ji
- K. Hwang
- Seockmo Ku
- PubMed: 33757506
- DOI: 10.1186/s12934-021-01563-w
Abstract
Background: Lactobacillus spp. have been researched worldwide and are used in probiotics, but due to difficulties with laboratory cultivation of and experimentation on oral microorganisms, there are few reports of Lactobacillus spp. being isolated from the oral cavity and tested against oral pathogens. This research sought to isolate and determine the safety and inhibitory capabilities of a Lactobacillus culture taken from the human body.
Results: One organism was isolated, named "L. gasseri HHuMIN D", and evaluated for safety. A 5% dilution of L. gasseri HHuMIN D culture supernatant exhibited 88.8% inhibition against halitosis-producing anaerobic microorganisms and the organism itself exhibited powerful inhibitory effects on the growth of 11 oral bacteria. Hydrogen peroxide production reached 802 μmol/L after 12 h and gradually diminished until 24 h, it efficiently aggregated with P. catoniae and S. sanguinis, and it completely suppressed S. mutans-manufactured artificial dental plaque. L. gasseri HHuMIN D's KB cell adhesion capacity was 4.41 cells per cell, and the cell adhesion of F. nucleatum and S. mutans diminished strongly in protection and displacement assays.
Conclusion: These results suggest that L. gasseri HHuMIN D is a safe, bioactive, lactobacterial food ingredient, starter culture, and/or probiotic microorganism for human oral health.
Keywords: Antimicrobial Effect; Biosafety Evaluation; Lactobacillus gasseri; Oral Microorganisms.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus gasseri | Increased Hydrogen Peroxide Production | Beneficial | Moderate |
| Lactobacillus gasseri | Reduced Formation of Dental Plaque | Beneficial | Large |
| Lactobacillus gasseri | Reduced Halitosis | Beneficial | Large |
| Lactobacillus gasseri | Reduced Oral Carriage of Cariogenic Pathogens | Beneficial | Large |
| Lactobacillus gasseri KS-13 | Increased Bacterial Aggregation | Beneficial | Moderate |
| Lactobacillus gasseri KS-13 | Increased Hydrogen Peroxide Production | Beneficial | Moderate |
| Lactobacillus gasseri KS-13 | Reduced Cell Adhesion | Beneficial | Moderate |
| Lactobacillus gasseri KS-13 | Reduced Formation of Dental Plaque | Beneficial | Large |
| Lactobacillus gasseri KS-13 | Reduced Halitosis | Beneficial | Large |
| Lactobacillus gasseri KS-13 | Reduced Oral Bacteria Growth | Beneficial | Large |
| Lactobacillus gasseri LAC-343 | Enhanced Cell Adhesion Capacity | Beneficial | Moderate |
| Lactobacillus gasseri LAC-343 | Increased Hydrogen Peroxide Production | Beneficial | Moderate |
| Lactobacillus gasseri LAC-343 | Reduced Antimicrobial Aggregation | Beneficial | Moderate |
| Lactobacillus gasseri LAC-343 | Reduced Formation of Dental Plaque | Beneficial | Large |
| Lactobacillus gasseri LAC-343 | Reduced Halitosis | Beneficial | Large |
| Lactobacillus gasseri LAC-343 | Reduced Oral Bacteria Growth | Beneficial | Large |
| Lactobacillus gasseri LQ-36 | Enhanced Adhesion Capacity to Oral Cells | Beneficial | Moderate |
| Lactobacillus gasseri LQ-36 | Increased Bacterial Aggregation | Beneficial | Moderate |
| Lactobacillus gasseri LQ-36 | Increased Hydrogen Peroxide Production | Beneficial | Moderate |
| Lactobacillus gasseri LQ-36 | Reduced Formation of Dental Plaque | Beneficial | Large |
| Lactobacillus gasseri LQ-36 | Reduced Halitosis | Beneficial | Large |
| Lactobacillus gasseri LQ-36 | Reduced Oral Bacteria Growth | Beneficial | Large |
| Lactobacillus gasseri LQ-36 | Reduced Pathogenic Bacteria Adhesion | Beneficial | Large |