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Pediococcus pentosaceus PR-1 modulates high-fat-died-induced alterations in gut microbiota, inflammation, and lipid metabolism in zebrafish

  • 2023-02-01
  • Frontiers in Nutrition 10
    • Yue Liu
    • D-L Zhu
    • Jiwen Liu
    • X. Sun
    • Feng Gao
    • Hui-Ping Duan
    • Lina Dong
    • Xin Wang
    • Changxing Wu

Abstract

Introduction: Obesity is a health issue worldwide. This study aimed to evaluate the beneficial effects of Pediococcus pentococcus PR-1 on the modulating of gut microbiota, inflammation and lipid metabolism in high-fat-diet (HFD)-fed zebrafish.

Methods: Adult zebrafish were fed a commercial (C), high fat (H, 25% fat), probiotic (P, 106 CFU/g), or high fat with probiotic (HP) diets twice daily for 5 weeks. Gut microbiota were analysed using 16S rRNA gene sequencing. Gene expressions of intestinal cytokine, intestinal TJ protein, and liver lipid metabolism were analysed by quantitative real-time polymerase chain reaction. Biochemical and histological analysis were also performed.

Results and discussion: P. pentosaceus PR-1 reduced body weight and BMI, indicating its anti-obesity effect. The 16S rRNA sequencing results showed HFD induced a distinct gut microbiota structure from C group, which was restored by probiotic. P. pentosaceus PR-1 improved gut health by decreasing the abundance of Ralstonia and Aeromonas which were increased induced by HFD. Moreover, probiotic restored abundance of Fusobacteria, Cetobacterium and Plesiomonas, which were decreased in HFD-fed zebrafish. The results of quantitative real-time polymerase chain reaction showed probiotic suppressed HFD-induced inflammation by decreasing the expressions of IL-1b and IL-6. Levels of hepatic TNF-α, IL-1ß, and IL-6 were reduced by probiotic in HFD-fed zebrafish. Probiotic also ameliorated gut barrier function by increasing the expressions of occludin, Claudin-1, and ZO-1. Probiotic exerted anti-adipogenic activity through regulating the expressions of SREBP1, FAS and LEPTIN. Levels of hepatic triglyceride, total cholesterol, low density lipoprotein were also reduced by probiotic. Histological analysis showed probiotic alleviated liver steatosis and injury induced by HFD. P. pentosaceus PR-1 might be useful as a dietary health supplement, especially for reducing obesity.

Keywords: gut microbiota; high fat diet; inflammation; probiotic; zebrafish.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Pediococcus pentosaceus KABP™-041Improved Blood Lipid MetabolismBeneficial
Moderate
Pediococcus pentosaceus KABP™-041Improved Intestinal Barrier FunctionBeneficial
Moderate
Pediococcus pentosaceus KABP™-041Increased Beneficial Gut BacteriaBeneficial
Moderate
Pediococcus pentosaceus KABP™-041Reduced Harmful Gut BacteriaBeneficial
Moderate
Pediococcus pentosaceus KABP™-041Reduced InflammationBeneficial
Large
Pediococcus pentosaceus KABP™-041Restored Gut Microbiota StructureBeneficial
Large
Pediococcus pentosaceus MAK99P15PImproved Gut MicrobiomeBeneficial
Moderate
Pediococcus pentosaceus MAK99P15PImproved Gut Microbiome CompositionBeneficial
Moderate
Pediococcus pentosaceus MAK99P15PImproved Intestinal Barrier FunctionBeneficial
Moderate
Pediococcus pentosaceus MAK99P15PReduced Body WeightBeneficial
Moderate
Pediococcus pentosaceus MAK99P15PReduced Inflammation LevelsBeneficial
Large
Pediococcus pentosaceus PP06Enhanced Intestinal Barrier FunctionBeneficial
Moderate
Pediococcus pentosaceus PP06Improved Blood Lipid MetabolismBeneficial
Moderate
Pediococcus pentosaceus PP06Improved Liver SteatosisBeneficial
Moderate
Pediococcus pentosaceus PP06Increased Abundance of Beneficial Gut GeneraBeneficial
Moderate
Pediococcus pentosaceus PP06Reduced Abundance of Harmful BacteriaBeneficial
Moderate
Pediococcus pentosaceus PP06Reduced Body WeightBeneficial
Moderate
Pediococcus pentosaceus PP06Reduced Inflammatory CytokinesBeneficial
Moderate
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