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Physicochemical, bioactive and rheological properties of an exopolysaccharide produced by a probiotic Pediococcus pentosaceus M41.

  • 2020-02
  • Carbohydrate Polymers 229
    • M. Ayyash
    • B. Abu-Jdayil
    • A. Olaimat
    • G. Esposito
    • P. Itsaranuwat
    • T. Osaili
    • R. Obaid
    • Jaleel Kizhakkayil
    • Shaoquan Liu

Abstract

This study aimed to investigate the physicochemical properties, health-promoting benefits and rheological properties of an EPS produced by a novel probiotic Pediococcus pentosaceus M41 isolated from a marine source. P. pentosaceus M41 was able to produce an EPS with average molecular weight of 682.07 kDa. EPS-M41 consisted of arabinose, mannose, glucose and galactose with a molar ratio of 1.2:1.8:15.1:1.0. EPS-M41 structure could be proposed as →3)α-D-Glc(1→2)β-D-Man(1→2)α-D-Glc(1→6)α-D-Glc(1→4)α-D-Glc(1→4)α-D-Gal(1→ with arabinose linked at the terminals. At concentration of 10 mg.ml-1, the antioxidant capacity was 76.5% and 48.9% for DPPH and ABTS, respectively. EPS-M41 inhibited 86.8% and 90.8% of the α-amylase and α-glucosidase activities, respectively, at 100 μg.ml-1. A 77.5% and 46.4% of antitumor inhibition occurred by EPS-M41 against Caco-2 and MCF-7 cells, respectively. The apparent viscosity (ƞ) of all EPS-M41 solutions decreased with shear rate increases. Salt type and pH value had an impact on the rheological properties of EPS-M41.

Keywords: 1,1-diphenyl-2-picrylhydrazyl DPPH (PubChem CID: 2735032); Antidiabetic; Antioxidants; Antitumor; D-Arabinose (PubChem CID:439195); D-Galactose (PubChem CID:6036); D-Glucose (PubChem CID:5793); Deuterium oxide (PubChem CID:24602); Exopolysaccharides; Pediococcus pentosaceus; Rheological properties; Trifluoroacetic acid (PubChem CID:6422); p-nitrophenyl ⍺-D-glucopyranoside (PubChem CID: 102764).

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Pediococcus pentosaceus KABP™-041Decreased Tumor Activity in Caco-2 CellsBeneficial
Large
Pediococcus pentosaceus KABP™-041Increased Antioxidant CapacityBeneficial
Large
Pediococcus pentosaceus KABP™-041Inhibited α-Amylase ActivityBeneficial
Large
Pediococcus pentosaceus KABP™-041Reduced Tumor Activity against MCF-7 CellsBeneficial
Moderate
Pediococcus pentosaceus KABP™-041Reduced α-Glucosidase ActivityBeneficial
Large
Pediococcus pentosaceus MAK99P15PDecreased Tumor Activity in Caco-2 CellsBeneficial
Large
Pediococcus pentosaceus MAK99P15PImproved Antioxidant CapacityBeneficial
Large
Pediococcus pentosaceus MAK99P15PReduced Alpha-Amylase ActivityBeneficial
Large
Pediococcus pentosaceus MAK99P15PReduced Tumor Activity against MCF-7 CellsBeneficial
Moderate
Pediococcus pentosaceus MAK99P15PReduced α-Glucosidase ActivityBeneficial
Large
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