Preparation of spent brewer's yeast beta-glucans with a potential application as an immunostimulant for black tiger shrimp, Penaeus monodon.
- 2003-05
- Bioresource Technology 88(1)
- M. Suphantharika
- P. Khunrae
- P. Thanardkit
- C. Verduyn
- PubMed: 12573564
- DOI: 10.1016/S0960-8524(02)00257-2
Abstract
Spent brewer's yeast was autolysed and the insoluble yeast cell wall fraction obtained was used as a raw material for the preparation of brewer's yeast beta-glucan (BYG). A simple alkaline extraction was applied and optimized. The BYG with significantly (P</=0.05) high carbohydrate and glucan contents of 92% and 51% (w/w), respectively and with a low protein content of 1.6% (w/w) was obtained by a single alkaline extraction using 5 volumes of 1.0 N NaOH at 90 degrees C for 1 h. In vitro, this glucan significantly (P</=0.05) enhanced phenoloxidase (PO) activity of black tiger shrimp hemolymph as compared to controls without added glucan. Also in vivo, an oral administration of 0.2% (w/w) in diets for 3 days significantly (P</=0.05) increased the PO-activity of the shrimp. The potential immunostimulating properties of a commercial and other yeast-derived products were also evaluated for comparative purposes.
Research Insights
Supplement | Health Outcome | Effect Type | Effect Size |
---|---|---|---|
Brewer's Yeast | Enhanced Phenoloxidase Activity | Beneficial | Moderate |