Prevention of High-Fat Diet-Induced Hypercholesterolemia by Lactobacillus reuteri Fn041 Through Promoting Cholesterol and Bile Salt Excretion and Intestinal Mucosal Barrier Functions
- 2022-03-11
- Frontiers in Nutrition 9
- Mengyao Lu
- Jin Sun
- Yuning Zhao
- Haowen Zhang
- Xinyue Li
- Jingbo Zhou
- Hongyang Dang
- Jidong Zhang
- Wenjing Huang
- Ce Qi
- Duo Li
- PubMed: 35369106
- DOI: 10.3389/fnut.2022.851541
Study Design
- Type
- Clinical Trial
- Population
- C57BL/6N mice
- Methods
- In vivo experiment with mice.
- Animal Study
Abstract
Objectives: Lactobacillus reuteri Fn041 (Fn041) is a probiotic isolated from immunoglobulin A coated microbiota in the human breast milk of Gannan in China with a low incidence of hypercholesterolemia. This study aims to explore the role and mechanism of Fn041 in preventing hypercholesterolemia caused by a high-fat diet in mice.
Methods: C57BL/6N mice were fed a low-fat diet or a high-fat diet and gavage with Fn041 and Lactobacillus rhamnosus GG (LGG) for 8 weeks.
Results: Both Fn041 and LGG prevented the occurrence of hypercholesterolemia, liver and testicular fat accumulation. In addition, a high-fat diet causes intestinal dysbiosis and mucosal barrier damage, which is associated with hypercholesterolemia. Fn041 prevented the high-fat diet-induced reduction in alpha diversity of intestinal microbiota and intestinal mucosal barrier damage. Fn041 treatment significantly increased fecal total cholesterol and total bile acids.
Conclusions: Fn041 prevented hypercholesterolemia by enhancing cholesterol excretion and mucosal barrier function.
Keywords: Lactobacillus reuteri; bile acid; cholesterol; gut microbiota; high-fat diet.