Probiotic and antioxidant properties of noble Lactobacillus brevis KCCM 12203P isolated from kimchi and evaluation of immune-stimulating activities of its heat-killed cells in RAW 264.7 cells.
- 2019-12-28
- Journal of Microbiology and Biotechnology 29(12)
- M. Song
- H. Jang
- Kee-Tae Kim
- H. Paik
- PubMed: 31581386
- DOI: 10.4014/jmb.1907.07081
Abstract
The purpose of this study was to determine the probiotic properties of Lactobacillus brevis KCCM 12203P isolated from the Korean traditional food kimchi and to evaluate the antioxidative activity and immune-stimulating potential of its heat-killed cells to improve their bio-functional activities. Lactobacillus rhamnosus GG, which is a representative commercial probiotic, was used as a comparative sample. Regarding probiotic properties, L. brevis KCCM 12203P was resistant to 0.3% pepsin with a pH of 2.5 for 3 h and 0.3% oxgall solution for 24 h, having approximately a 99% survival rate. It also showed strong adhesion activity (6.84%) onto HT-29 cells and did not produce β-glucuronidase but produced high quantities of leucine arylamidase, valine arylamidase, β-galactosidase, and N-acetyl-β- glucosaminidase. For antioxidant activity, it appeared that viable cells had higher radical scavenging activity in the 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assay, while in the 2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay, heat-killed cells had higher antioxidant activity. Additionally, L. brevis KCCM 12203P showed higher lipid oxidation inhibition ability than L. rhamnosus GG; however, there was no significant difference (p < 0.05) between heat-killed cells and control cells. Furthermore, heat-killed L. brevis KCCM 12203P activated RAW 264.7 macrophage cells without cytotoxicity at a concentration lower than 108 CFU/ml and promoted higher gene expression levels of inducible nitric oxide synthase, interleukin-1β, and interleukin-6 than L. rhamnosus GG. These results suggest that novel L. brevis KCCM 12203P could be used as a probiotic or applied to functional food processing and pharmaceutical fields for immunocompromised people.
Keywords: Lactobacillus brevis; antioxidant activity; heat-killed bacteria; immune-stimulating activity; probiotics.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus brevis | Enhanced Antioxidant Activity | Beneficial | Moderate |
| Lactobacillus brevis | Improved Probiotic Function | Beneficial | Large |
| Lactobacillus brevis | Increased Immune System Activation | Beneficial | Large |
| Lactobacillus brevis | Reduced Lipid Oxidation | Beneficial | Moderate |
| Lactobacillus brevis HA-112 | Enhanced Immune Function | Beneficial | Large |
| Lactobacillus brevis HA-112 | Improved Antioxidant Activity | Beneficial | Moderate |
| Lactobacillus brevis KABP™-052 | Enhanced Antioxidant Activity | Beneficial | Moderate |
| Lactobacillus brevis KABP™-052 | Enhanced Immune Response via Gene Expression | Beneficial | Large |
| Lactobacillus brevis KABP™-052 | Enhanced Intestinal Cell Adhesion | Beneficial | Large |
| Lactobacillus brevis KABP™-052 | Improved Survival in Gastrointestinal Conditions | Beneficial | Large |
| Lactobacillus brevis KABP™-052 | Increased Enzyme Production | Beneficial | Moderate |
| Lactobacillus brevis KABP™-052 | Increased Viable Cell Antioxidant Activity | Beneficial | Large |
| Lactobacillus brevis KABP™-052 | Inhibited Lipid Oxidation | Beneficial | Moderate |
| Lactobacillus brevis LB01 | Enhanced Immune Function | Beneficial | Large |
| Lactobacillus brevis LB01 | Enhanced Probiotic Resilience | Beneficial | Large |
| Lactobacillus brevis LB01 | Improved Intestinal Cell Adhesion | Beneficial | Large |
| Lactobacillus brevis LB01 | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus brevis LB01 | Reduced Lipid Oxidation | Beneficial | Moderate |
| Lactobacillus brevis Lbr-35 | Enhanced Intestinal Cell Adhesion | Beneficial | Large |
| Lactobacillus brevis Lbr-35 | Improved Survival Rate in Digestive Conditions | Beneficial | Large |
| Lactobacillus brevis Lbr-35 | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus brevis Lbr-35 | Increased Immune System Activation | Beneficial | Large |
| Lactobacillus brevis Lbr-35 | Increased Inhibition of Lipid Oxidation | Beneficial | Moderate |
| Lactobacillus brevis SBC8803 | Improved Survival in Gastrointestinal Conditions | Beneficial | Large |
| Lactobacillus brevis SBC8803 | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus brevis SBC8803 | Increased Enzyme Production | Beneficial | Moderate |
| Lactobacillus brevis SBC8803 | Increased Immune Activation without Cytotoxicity | Beneficial | Large |
| Lactobacillus brevis SBC8803 | Inhibited Lipid Oxidation | Beneficial | Moderate |
| Lactobacillus brevis SBC8803 | Reduced Intestinal Cell Adhesion | Beneficial | Moderate |
| Lactobacillus brevis SD-5214 | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus brevis SD-5214 | Increased Immune Response | Beneficial | Moderate |
| Lactobacillus brevis SD-5214 | Increased Resistance to Gastrointestinal Conditions | Beneficial | Large |
| Lactobacillus brevis SD-5214 | Increased Viable Cell Antioxidant Activity | Beneficial | Large |
| Lactobacillus brevis SD-5214 | Inhibited Lipid Oxidation | Beneficial | Moderate |
| Lactobacillus brevis SD-5214 | Reduced Adhesion to Human Intestinal Cells | Beneficial | Moderate |
| Lactobacillus brevis UALbr-02 | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus brevis UALbr-02 | Increased Immune Factor Levels | Beneficial | Large |
| Lactobacillus brevis UALbr-02 | Inhibited Lipid Oxidation | Beneficial | Moderate |
| Lactobacillus brevis VPro 18 | Improved Gut Microbiota | Beneficial | Large |