Probiotic and antioxidant properties of selenium-enriched Lactobacillus brevis LSe isolated from an Iranian traditional dairy product.
- 2017-03
- Journal of Trace Elements in Medicine and Biology 40
- Mojtaba Shakibaie
- Tayebe Mohammadi-Khorsand
- Mahboubeh Adeli-sardou
- M. Jafari
- Sahar Amirpour-Rostami
- A. Ameri
- H. Forootanfar
- PubMed: 28159215
- DOI: 10.1016/j.jtemb.2016.11.013
Abstract
The present study was designed to isolate a highly selenium-tolerant lactobacillus strain from an Iranian traditional dairy product named as Spar. Different criteria such as tolerance to the low pH, simulated gastric juice (SGJ), simulated intestinal juice (SIJ) and bile salts tolerance as well as Caco-2 cell adhesion assay were examined to evaluate the probiotic potentials of the selected isolate. Furthermore, the antioxidant properties of the isolate cultivated in medium containing and free of SeO32- ions were evaluated using DPPH scavenging and reducing power assays. The isolate was identified using conventional identification and 16S rDNA gene sequencing methods as Lactobacillus brevis LSe. The obtained results showed that the isolate was able to tolerate high concentration of sodium selenite (3.16mM). By decreasing the pH of the SGJ from 6 to 3, the survival percent of L. brevis LSe was not significantly changed over the time (p>0.05). In addition, the survival percent of the isolate in the SIJ (pH 6 and pH 8) was not statistically altered after 3h, 6h and 24h of incubation (p>0.05). In the presence of bile salts (0.3% and 0.6%) the survival rate of L. brevis LSe was not significantly decreased (p>0.05).L. brevis LSe also demonstrated the satisfactory ability to adhere to Caco-2 cells which were similar to that of the reference strain L. plantarum. The obtained results of antioxidant evaluation showed that L. brevis LSe containing elemental Se exhibited significantly higher radical scavenging ability (36.5±1.31%) and reducing power (OD700, 0.14) than L. brevis LSe cultured in selenite-free medium (p<0.05). To sum up, further investigations should be conducted to merit the probable potential health benefit of Se-enriched L. brevis LSe and its application as Se-containing supplements or fermented foods.
Keywords: 16S rDNA; Antioxidant; Lactobacillus brevis; Probiotic; Se-enriched lactobacillus.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus brevis | Enhanced Antioxidant Activity | Beneficial | Large |
| Lactobacillus brevis HA-112 | Improved Survival Through Gastric Conditions | Beneficial | Large |
| Lactobacillus brevis HA-112 | Improved Tolerance to Bile Salts | Beneficial | Large |
| Lactobacillus brevis HA-112 | Improved Tolerance to Intestinal Conditions | Beneficial | Large |
| Lactobacillus brevis HA-112 | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus brevis HA-112 | Reduced Intestinal Cell Adhesion | Beneficial | Large |
| Lactobacillus brevis KABP™-052 | Adhesion to Caco-2 Cells | Beneficial | Moderate |
| Lactobacillus brevis KABP™-052 | Enhanced Radical Scavenging Ability | Beneficial | Moderate |
| Lactobacillus brevis KABP™-052 | Gastrointestinal Survival | Beneficial | Large |
| Lactobacillus brevis KABP™-052 | Increased Bile Salt Tolerance | Beneficial | Large |
| Lactobacillus brevis KABP™-052 | Increased Selenium Tolerance | Beneficial | Large |
| Lactobacillus brevis LB01 | Enhanced Antioxidant Activity | Beneficial | Large |
| Lactobacillus brevis LB01 | Increased Bile Salt Tolerance | Beneficial | Large |
| Lactobacillus brevis LB01 | Increased Selenium Tolerance | Beneficial | Large |
| Lactobacillus brevis LB01 | Reduced Intestinal Cell Adhesion | Beneficial | Large |
| Lactobacillus brevis MAK11L82B | Adhesion to Caco-2 Cells | Beneficial | Moderate |
| Lactobacillus brevis MAK11L82B | Improved Resistance to Sodium Selenite | Beneficial | Moderate |
| Lactobacillus brevis MAK11L82B | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus brevis MAK11L82B | Maintained Cell Viability in Low pH | Beneficial | Moderate |
| Lactobacillus brevis MAK11L82B | Maintained Viability in Presence of Bile Salts | Beneficial | Moderate |
| Lactobacillus brevis MAK11L82B | Maintained Viability in Sacroiliac Joint | Beneficial | Moderate |
| Lactobacillus brevis SD-5214 | Adhesion to Caco-2 Cells | Beneficial | Moderate |
| Lactobacillus brevis SD-5214 | Enhanced Antioxidant Capabilities | Beneficial | Large |
| Lactobacillus brevis SD-5214 | Increased Sodium Selenite Tolerance | Beneficial | Large |
| Lactobacillus brevis UALbr-02 | Enhanced Probiotic Viability | Beneficial | Moderate |
| Lactobacillus brevis UALbr-02 | Improved Antioxidant Activity | Beneficial | Large |
| Lactobacillus brevis UALbr-02 | Improved Cell Adhesion | Beneficial | Moderate |