study.

2024-08-29
Veterinarni medicina 69(8)
- Iveta Kostovova
- Katerina Kavanova
- Monika Moravkova
- Jan Gebauer
- Lenka Leva
- Monika Vicenova
- Vladimir Babak
- Martin Faldyna
- Magdalena Crhanova

PubMed: 39296628
DOI: 10.17221/35/2024-vetmed

Using probiotics represents a potential solution to post-weaning diarrheal diseases in piglets on commercial farms. The gastrointestinal tract of wild boars serves as a promising reservoir of novel lactic acid bacteria with suitable probiotic characteristics. In this study, we isolated eight bacterial strains from the intestinal content of wild boars identified as representatives of the species Bifidobacterium apri, Lactobacillus amylovorus, and Ligilactobacillus salivarius. These isolates underwent in vitro analysis and characterisation to assess their biological safety and probiotic properties. Analysis of their full genome sequences revealed the absence of horizontally transferrable genes for antibiotic resistance. However, seven out of eight isolates harboured genes encoding various types of bacteriocins in their genomes, and bacteriocin production was further confirmed by mass spectrometry analysis. Most of the tested strains demonstrated the ability to inhibit the growth of selected pathogenic bacteria, produce exopolysaccharides, and stimulate the expression of interleukin-10 in porcine macrophages. These characteristics deem the isolates characterised in this study as potential candidates for use as probiotics for piglets during the post-weaning period.

Research Insights

Supplement	Health Outcome	Effect Type	Effect Size
Lactobacillus amylovorus	Increased Interleukin-10 Expression	Beneficial	Moderate
Lactobacillus amylovorus	Reduced Pathogen Growth	Beneficial	Moderate