Probiotic characterization of Lactobacillus brevis KU15153 showing antimicrobial and antioxidant effect isolated from kimchi
- 2019-03-04
- Food Science and Biotechnology 28(5)
- H. Jang
- N. Lee
- H. Paik
- PubMed: 31695951
- DOI: 10.1007/S10068-019-00576-X
Abstract
Lactobacillus brevis KU15153 was isolated from kimchi and probiotic characterization was performed including analysis of its antimicrobial and antioxidant effects. Lactobacillus rhamnosus GG (LGG) was used as a probiotic control. L. brevis KU15153 survived under artificial gastric conditions and was non-hemolytic, showed antibiotic susceptibility, and did not produce carcinogenic β-glucuronidase. L. brevis KU15153 adhered strongly to HT-29 cells in the direct adherent assay and showed high cell surface hydrophobicity. Particularly, L. brevis KU15153 showed antimicrobial activity against the food-borne pathogens Escherichia coli ATCC 25922, Listeria monocytogenes ATCC 15313, Salmonella Typhimurium P99, and Staphylococcus aureus KCCM 11335. Antioxidant activity was assessed using the DPPH radical scavenging assay and β-carotene and linoleic acid inhibition assay. L. brevis KU15153 showed higher antioxidant activity than LGG. These results suggest that L. brevis KU15153 has potential for use as a probiotic organism.
Keywords: Antimicrobial effect; Antioxidant effect; Kimchi; Lactobacillus brevis; Probiotic.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus brevis | Improved Antimicrobial Activity Against Foodborne Pathogens | Beneficial | Large |
| Lactobacillus brevis | Improved Gastric Survival | Beneficial | Large |
| Lactobacillus brevis | Increased Antibiotic Susceptibility | Beneficial | Large |
| Lactobacillus brevis | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus brevis | Increased Cell Adhesion | Beneficial | Large |
| Lactobacillus brevis | Increased Cell Surface Hydrophobicity | Beneficial | Large |
| Lactobacillus brevis | Non-Hemolytic Blood Response | Beneficial | Large |
| Lactobacillus brevis | Reduced Production of Carcinogenic Enzymes | Beneficial | Large |
| Lactobacillus brevis UALbr-02 | Improved Antimicrobial Activity Against Foodborne Pathogens | Beneficial | Large |
| Lactobacillus brevis UALbr-02 | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus brevis UALbr-02 | Increased Intestinal Cell Adherence | Beneficial | Moderate |
| Lactobacillus brevis VPro 18 | Enhanced Antimicrobial Resistance | Beneficial | Large |
| Lactobacillus brevis VPro 18 | Increased Antioxidant Activity | Beneficial | Large |