Probiotic consumption can modify the resilience of periodontal tissues in rats under experimental periodontitis.
- 2022-12-27
- Journal of Periodontology 94(2)
- L. F. F. de Oliveira
- P. Silva
- S. Salvador
- E. Ervolino
- R. Casarin
- L. Figueiredo
- Milla Tavares Ricoldi
- S. L. D. de Souza
- F. Furlaneto
- M. Messora
- PubMed: 35690993
- DOI: 10.1002/JPER.21-0555
Abstract
Background: This study evaluated the effects of systemic administration of Bifidobacterium animalis subsp. lactis HN019 (B. lactis HN019) on experimental periodontitis (EP) in rats.
Methods: Thirty-two rats were allocated to groups C (control), C-HN019 (probiotic), EP (EP only), and EP-HN019 (EP+probiotic). From day 0, the animals of C-HN019 and EP-HN019 groups received B. lactis HN019 (1 × 109 CFU/ml) daily. On the 14th day, the animals of EP and EP-HN019 groups received silk ligature around mandibular molars. Animals were euthanized on the 28th day. Samples of oral biofilm, gingival tissues, blood serum, and mandible were obtained for microtomographic, histomorphometric, microbiological, and immunological analyses. Data were statistically analyzed (p < 0.05).
Results: Group EP-HN019 presented significantly less alveolar bone loss when compared with Group EP in histomorphometric and microtomographic analyses. In gingival tissue and serum, Group EP-HN019 presented lower proinflammatory/anti-inflammatory cytokines ratios than Group EP. Group EP-HN019 showed higher expression of beta-defensins and less TRAP-positive cells than Group EP. Group EP presented higher gene expression of Ifng and lower gene expression of Foxp3 when compared with Group EP-HN019 in gingival tissue. In oral biofilm, EP-HN019 group presented a lower percentage of species similar to Fusobacterium periodonticum and a higher percentage of species similar to Actinomyces gereneseriae, Actinomyces israelli, and Streptococcus gordonii when compared with Group EP. There was a significant increase of B. lactis HN019 after administration of probiotic therapy in oral biofilm of Group EP-HN019.
Conclusion: The consumption of B. lactis HN019 promotes a protective effect against alveolar bone loss by modifying local and systemic microbiological and immunoinflammatory parameters.
Keywords: bifidobacterium; host microbial interactions; immunology; periodontitis; rats.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Bifidobacterium animalis subsp. lactis HN019 | Increased Beta-defensins Expression | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis HN019 | Increased Bifidobacterium lactis HN019 in Oral Biofilm | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis HN019 | Reduced Alveolar Bone Resorption | Beneficial | Large |
| Bifidobacterium animalis subsp. lactis HN019 | Reduced Proinflammatory/Anti-inflammatory Cytokine Ratios | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis HN019 | Reduced TRAP-Positive Cell Count | Beneficial | Moderate |
| Bifidobacterium lactis HN019 | Altered Oral Microbiota | Beneficial | Moderate |
| Bifidobacterium lactis HN019 | Increase in Bifidobacterium lactis HN019 | Beneficial | Moderate |
| Bifidobacterium lactis HN019 | Increased Beta-defensins Expression | Beneficial | Moderate |
| Bifidobacterium lactis HN019 | Reduced Alveolar Bone Resorption | Beneficial | Large |
| Bifidobacterium lactis HN019 | Reduced IFN-γ Expression | Beneficial | Moderate |
| Bifidobacterium lactis HN019 | Reduced Proinflammatory/Anti-inflammatory Cytokine Ratios | Beneficial | Moderate |