Probiotic Lactobacillus fermentum KU200060 isolated from watery kimchi and its application in probiotic yogurt for oral health.

2020-10
Microbial Pathogenesis 147
- Sung-Min Lim
- N. Lee
- Kee-Tae Kim
- H. Paik

PubMed: 32758520
DOI: 10.1016/j.micpath.2020.104430

Study Design

Methods: Controlled experimental study

Abstract

Lactobacillus fermentum KU200060 was isolated from watery kimchi and its probiotic characteristics were evaluated, including tolerance to artificial gastric acid and bile salt, production of enzymes, ability to adhere to HT-29 cells, and antibiotic susceptibility. The antibacterial and antibiofilm effects of L. fermentum KU200060 against Streptococcus mutans KCTC 5316 were compared to those of Lactobacillus rhamnosus GG and Lactobacillus brevis KU15006. L. fermentum KU200060 demonstrated higher antibacterial activity and inhibition of biofilm formation by S. mutans than L. rhamnosus GG via inhibiting formation of water-insoluble glucan and related gene expression. In addition, L. fermentum KU200060 was applied as a probiotic in yogurt, and its physicochemical property and sensory value demonstrated its potential as a yogurt starter. The physicochemical characteristics and consumer acceptability of the probiotic yogurt containing L. fermentum KU200060 were not significantly different compared to those of the control yogurt. Therefore, L. fermentum KU200060 could be used for oral health in the probiotic industry.

Keywords: Anticavity activity; Oral health; Probiotic yogurt; Probiotics; Streptococcus mutans.

Research Insights

Lactobacillus fermentum LF61→Improved Antibacterial Activity
L. fermentum KU200060 demonstrated higher antibacterial activity and inhibition of biofilm formation by S. mutans than L. rhamnosus GG.
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum LF61→Improved Oral Health
L. fermentum KU200060 demonstrated higher antibacterial activity and inhibition of biofilm formation by S. mutans than L. rhamnosus GG via inhibiting formation of water-insoluble glucan and related gene expression.
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum MAK20L13F→Reduced Streptococcus mutans Activity
L. fermentum KU200060 demonstrated higher antibacterial activity and inhibition of biofilm formation by S. mutans than L. rhamnosus GG via inhibiting formation of water-insoluble glucan and related gene expression.
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum UALf-32→Potential Probiotic Benefits
In addition, L. fermentum KU200060 was applied as a probiotic in yogurt, and its physicochemical property and sensory value demonstrated its potential as a yogurt starter. The physicochemical characteristics and consumer acceptability of the probiotic yogurt containing L. fermentum KU200060 were not significantly different compared to those of the control yogurt.
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum UALf-32→Reduced Streptococcus mutans Activity
L. fermentum KU200060 demonstrated higher antibacterial activity and inhibition of biofilm formation by S. mutans than L. rhamnosus GG via inhibiting formation of water-insoluble glucan and related gene expression.
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum VPro 14→Reduced Mutans Streptococcus Counts
L. fermentum KU200060 demonstrated higher antibacterial activity and inhibition of biofilm formation by S. mutans than L. rhamnosus GG via inhibiting formation of water-insoluble glucan and related gene expression.
Effect
Beneficial
Effect size
Large