Probiotic Properties of Lactobacillus brevis KU200019 and Synergistic Activity with Fructooligosaccharides in Antagonistic Activity against Foodborne Pathogens
- 2020-03
- Food Science of Animal Resources 40(2)
- K. M. G. M. M. Kariyawasam
- Seo-Jin Yang
- N. Lee
- H. Paik
- PubMed: 32161924
- DOI: 10.5851/kosfa.2020.e15
Abstract
This study aims to evaluate the probiotic properties of Lactobacillus brevis (L. brevis) KU200019 and the synergistic activity with prebiotics on antimicrobial activity, and the potential application as an adjunct culture in fermented dairy products. The commercial strain, L. brevis ATCC 14869 was used as reference strain. L. brevis KU200019 was showed higher viability in simulated gastric (99.38±0.21%) and bile (115.10±0.13%) conditions compared to reference strain. L. brevis KU200019 exhibited antimicrobial activity against various foodborne pathogens. The supplementation of fructooligosaccharides (FOS) enhanced viability of lactic acid bacteria (>8 Log CFU/mL) and antioxidant activity [2,2-diphenyl-2-picrylhydrazyl radical assay (DPPH) assay, 31.23±1.14%; 2,2-azinobis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS) assay, 38.82±1.46%] in fermented skim milk during refrigerated storage. L. brevis KU200019 was distinguished from the reference strain by its higher probiotic potential, antimicrobial activity, and higher antioxidant activity in fermented milk. Therefore, L. brevis KU200019 with FOS was demonstrated promising properties for further application in fermented dairy products with enhanced safety and quality.
Keywords: antimicrobial activity; antioxidant activity; dairy application; probiotics; synbiotic.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus brevis | Enhanced Antimicrobial Activity | Beneficial | Large |
| Lactobacillus brevis | Increased Antioxidant Activity | Beneficial | Large |
| Lactobacillus brevis | Increased Lactic Acid Bacteria Levels | Beneficial | Large |
| Lactobacillus brevis KABP™-052 | Enhanced Antioxidant Activity | Beneficial | Moderate |
| Lactobacillus brevis KABP™-052 | Enhanced Gut Flora from Fermented Milk | Beneficial | Large |
| Lactobacillus brevis KABP™-052 | Improved Antimicrobial Activity Against Foodborne Pathogens | Beneficial | Large |
| Lactobacillus brevis KABP™-052 | Increased Lactic Acid Bacteria Levels | Beneficial | Large |
| Lactobacillus brevis LB01 | Improved Antimicrobial Activity Against Foodborne Pathogens | Beneficial | Large |
| Lactobacillus brevis LB01 | Improved Antioxidant Activity | Beneficial | Moderate |
| Lactobacillus brevis LB01 | Improved Digestive Viability | Beneficial | Large |
| Lactobacillus brevis LB01 | Improved Viability of Lactic Acid Bacteria | Beneficial | Large |
| Lactobacillus brevis Lbr-35 | Enhanced Antimicrobial Activity | Beneficial | Large |
| Lactobacillus brevis Lbr-35 | Improved Antioxidant Activity | Beneficial | Moderate |
| Lactobacillus brevis Lbr-35 | Improved Viability of Lactic Acid Bacteria | Beneficial | Large |
| Lactobacillus brevis Lbr-35 | Increased Probiotic Potential | Beneficial | Large |
| Lactobacillus brevis VPro 18 | Enhanced Gastric Viability | Beneficial | Large |
| Lactobacillus brevis VPro 18 | Enhanced Viability in Bile Conditions | Beneficial | Large |
| Lactobacillus brevis VPro 18 | Improved Antimicrobial Activity Against Foodborne Pathogens | Beneficial | Large |
| Lactobacillus brevis VPro 18 | Improved Viability of Lactic Acid Bacteria | Beneficial | Large |
| Lactobacillus brevis VPro 18 | Increased Antioxidant Activity | Beneficial | Large |