Prophylactic effects of oral administration of Lactobacillus casei on house dust mite-induced asthma in mice.
- 2020
- Food & Function 11(10)
- Lingzhi Li
- Zhifeng Fang
- Yuan-Kun Lee
- Jianxin Zhao
- Hao Zhang
- Wenwei Lu
- Wei Chen
- PubMed: 33047743
- DOI: 10.1039/d0fo01363c
Abstract
This study investigated the prophylactic effects of five Lactobacillus casei strains on house dust mite (HDM)-induced asthma in mice. BALB/c mice were orally administered with L. casei strains for one week before HDM treatment. Ketotifen and Lactobacillus rhamnosus GG were used as positive controls. All L. casei strains decreased the number of granulocytes and the levels of Th2 and Th17 inflammatory cytokines in the lungs, L. casei3 significantly decreased the airway inflammation score. Further studies showed that L. casei3, L. casei4, and L. casei5 decreased the chemokine levels, L. casei2, L. casei4, and L. casei5 promoted the secretion of secretory immunoglobulin A (sIgA), L. casei2 upregulated the interleukin (IL)-10 levels, and L. casei1 had no effect on these immune indices. L. casei1 and L. casei4 decreased the serum levels of total IgE and HDM-specific IgG1, respectively. L. casei3 and L. casei5 decreased both HDM-specific IgG1 and total IgE levels. L. casei2 did not affect the levels of these immunoglobulins. The gut microbiota analysis revealed that all five L. casei strains enhanced the richness of the gut microbiota mainly by increasing the abundance of Firmicutes, while there were differences at the genus level.Thus, the prophylactic effects of L. casei on HDM-induced mixed chronic airway inflammatory asthma exerted as they differentially affected the immune responses and gut microbiota composition. L. casei3, which exhibited the highest prophylactic effect, increased the acetate and propionate contents in a strain-dependent manner.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lacticaseibacillus casei | Enhanced Gut Microbiota Diversity | Beneficial | Moderate |
| Lacticaseibacillus casei | Increased Interleukin-10 Levels | Beneficial | Moderate |
| Lacticaseibacillus casei | Increased Secretory Immunoglobulin A (sIgA) | Beneficial | Moderate |
| Lacticaseibacillus casei | Reduced Airway Inflammation Score | Beneficial | Large |
| Lacticaseibacillus casei | Reduced Chemokine Levels | Beneficial | Moderate |
| Lacticaseibacillus casei | Reduced HDM-Specific IgG1 Levels | Beneficial | Moderate |
| Lacticaseibacillus casei | Reduced Total IgE Levels | Beneficial | Moderate |
| Lacticaseibacillus casei R0215 | Improved Gut Microbial Richness | Beneficial | Large |
| Lacticaseibacillus casei R0215 | Increased Interleukin-10 Levels | Beneficial | Moderate |
| Lacticaseibacillus casei R0215 | Increased Secretory Immunoglobulin A | Beneficial | Moderate |
| Lacticaseibacillus casei R0215 | Reduced Airway Inflammation | Beneficial | Large |
| Lacticaseibacillus casei R0215 | Reduced Chemokine Levels | Beneficial | Moderate |
| Lacticaseibacillus casei R0215 | Reduced Granulocyte Levels | Beneficial | Large |
| Lactobacillus casei | Enhanced Gut Microbiota Diversity | Beneficial | Moderate |
| Lactobacillus casei | Increased Interleukin-10 Levels | Beneficial | Moderate |
| Lactobacillus casei | Increased Secretory Immunoglobulin A | Beneficial | Moderate |
| Lactobacillus casei | Reduced Airway Inflammation Score | Beneficial | Large |
| Lactobacillus casei | Reduced Chemokine Levels | Beneficial | Moderate |
| Lactobacillus casei | Reduced Granulocyte Levels | Beneficial | Large |
| Lactobacillus casei | Reduced HDM-Specific IgG1 Levels | Beneficial | Moderate |
| Lactobacillus casei | Reduced Total IgE Levels | Beneficial | Moderate |
| Lactobacillus casei R0215 | Improved Gut Microbiota Richness | Beneficial | Large |
| Lactobacillus casei R0215 | Increased Interleukin-10 Levels | Beneficial | Moderate |
| Lactobacillus casei R0215 | Increased Secretory Immunoglobulin A | Beneficial | Moderate |
| Lactobacillus casei R0215 | Reduced Airway Inflammation | Beneficial | Large |
| Lactobacillus casei R0215 | Reduced Chemokine Levels | Beneficial | Moderate |
| Lactobacillus casei R0215 | Reduced Granulocyte Levels | Beneficial | Large |
| Lactobacillus casei R0215 | Reduced HDM-Specific IgG1 Levels | Beneficial | Moderate |
| Lactobacillus casei R0215 | Reduced Total IgE Levels | Beneficial | Moderate |
| Lactobacillus casei VK4 | Decreased Granulocyte Count in Lungs | Beneficial | Moderate |
| Lactobacillus casei VK4 | Improved Gut Microbiota Richness | Beneficial | Large |
| Lactobacillus casei VK4 | Increased Secretory IgA Level | Beneficial | Moderate |
| Lactobacillus casei VK4 | Reduced Airway Inflammation | Beneficial | Large |
| Lactobacillus casei VK4 | Reduced Chemokine Levels | Beneficial | Moderate |
| Lactobacillus casei VK4 | Reduced Inflammatory Cytokines | Beneficial | Moderate |