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Protective Effect of Lactobacillus diolivorans 1Z, Isolated From Brazilian Kefir, Against Salmonella enterica Serovar Typhimurium in Experimental Murine Models

  • 2018-12-04
  • Frontiers in Microbiology 9
    • Mário Abatemarco Júnior
    • S. Sandes
    • M. F. Ricci
    • R. Arantes
    • Á. Nunes
    • J. Nicoli
    • E. Neumann

Abstract

Kefir is a beverage obtained by fermentation of milk or sugar solution by lactic acid bacteria and yeasts, and several health benefits have been attributed to its ingestion, part of them being attributed to Lactobacillus species. The objective of the present study was to evaluate, in vivo, the probiotic potential of Lactobacillus diolivorans 1Z, isolated from Brazilian kefir grains. Initially, conventional mice were orally treated daily or not during 10 days with a suspension of L. diolivorans 1Z, and then orally challenged with Salmonella enterica serovar Typhimurium. Treatment with L. diolivorans 1Z resulted in higher survival (70%) of animals after the challenge with the pathogen than for not treated mice (0%). When germ-free mice were monoassociated (GN-PS group) or not (GN-CS group) with L. diolivorans 1Z and challenged after 7 days with S. Typhimurium, Salmonella fecal counts were significantly lower (P < 0.05) for the GN-PS group when compared to the GN-CS group. Histopathological analysis revealed less damage to the ileum mucosa, as demonstrated by smallest perimeter of major lesions for mice of the GN-PS group in comparison to the group GN-CS (P < 0.05). These findings were accompanied by a lower expression of IFN-γ and TNF-α in the intestinal tissue of GN-PS mice. Additionally, translocation of S. Typhimurium to liver was significantly lower in GN-PS than in GN-CS mice (P < 0.05), and IgA levels in intestinal content and number of Kupffer cells in liver were higher. No difference was observed for hepatic cellularity between GN-PS and GN-CS groups (P > 0.05), but the pattern of inflammatory cells present in the liver was predominantly of polymorphonuclear in GN-CS group and of mononuclear in the GN-PS group, and a higher hepatic expression of IL-10 and TGF-β was observed in GN-PS group. Concluding, L. diolivorans 1Z showed to be a potential probiotic strain that protected mice from death after challenge with S. Typhimurium, apparently by immunological modulation.

Keywords: Lactobacillus diolivorans; Salmonella Typhimurium; gnotobiotic mice; kefir; probiotic.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus kefirIncreased Intestinal IgA LevelsBeneficial
Small
Lactobacillus kefirIncreased Survival After Salmonella Typhimurium InfectionBeneficial
Large
Lactobacillus kefirModified Hepatic Inflammatory Cell PatternBeneficial
Small
Lactobacillus kefirReduced Fecal Salmonella BacteriaBeneficial
Moderate
Lactobacillus kefirReduced Ileum Mucosa DamageBeneficial
Moderate
Lactobacillus kefirReduced Salmonella Translocation to LiverBeneficial
Moderate
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