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Regulatory effect of paraprobiotic Lactobacillus gasseri CP2305 on gut environment and function

  • 2016-03-14
  • Microbial Ecology in Health & Disease 27(0)
    • Tomonori Sugawara
    • D. Sawada
    • Y. Ishida
    • Kotaro Aihara
    • Yumeko Aoki
    • Isao Takehara
    • Kazuhiko Takano
    • S. Fujiwara

Abstract

Background: Lactobacillus gasseri CP2305 (CP2305) is a strain of Lactobacillus isolated from a stool sample from a healthy adult that showed beneficial effects on health as a paraprobiotic. In a previous study, we demonstrated that CP2305-fermented heat-treated milk modified gut functions more than artificially acidified sour milk. Thus, the regulatory activity of the former beverage was attributed to the inactivated CP2305 cells.

Objective: The aim of this study was to elucidate the contribution of non-viable paraprobiotic CP2305 cells to regulating human gut functions. We thus conducted a randomized, placebo-controlled, double-blinded parallel group trial.

Design: The trial included 118 healthy participants with relatively low or high stool frequencies. The test beverage was prepared by adding 1×10(10) washed, heat-treated, and dried CP2305 cells directly to the placebo beverage. The participants ingested a bottle of the assigned beverage daily for 3 weeks and answered daily questionnaires about defecation and quality of life. Fecal samples were collected and the fecal characteristics, microbial metabolite contents of the feces and composition of fecal microbiota were evaluated.

Results: The number of evacuations and the scores for fecal odors were significantly improved in the group that consumed the CP2305-containing beverage compared with those of the group that consumed the placebo (p=0.035 and p=0.040, respectively). Regarding the fecal contents of microbial metabolites, the level of fecal p-cresol was significantly decreased in the CP2305 group relative to that of the placebo group (p=0.013). The Bifidobacterium content of the intestinal microbiota was significantly increased in the CP2305 group relative to that of the placebo group (p<0.008), whereas the content of Clostridium cluster IV was significantly decreased (p<0.003). The parasympathetic nerve activity of the autonomic nervous system became dominant and the total power of autonomic activity was elevated in the CP2305 group (p=0.0401 and p=0.011, respectively).

Conclusions: The continuous ingestion of heat-treated CP2305 cells clearly affected intestinal functionality. This is the first report of sterilized Lactobacillus cells having a significant impact on the environment and functions of the intestinal tract. The observed effects might be due, at least in part, to the brain-gut interaction.

Keywords: abdominal symptoms; bacterial flora; defecation habits; heat-inactivated lactic acid-producing bacilli; intraluminal environment; parasympathetic activation.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus gasseriEnhanced Parasympathetic Nervous System ActivityBeneficial
Moderate
Lactobacillus gasseriImproved Stool FrequencyBeneficial
Moderate
Lactobacillus gasseriIncreased Bifidobacterium Levels in FecesBeneficial
Large
Lactobacillus gasseriReduced Clostridium Cluster IV ContentBeneficial
Large
Lactobacillus gasseriReduced Fecal p-Cresol LevelsBeneficial
Large
Lactobacillus gasseri BNR17Enhanced Autonomic Activity Total PowerBeneficial
Large
Lactobacillus gasseri BNR17Improved Bowel Movement FrequencyBeneficial
Moderate
Lactobacillus gasseri BNR17Increased Bifidobacterium Levels in FecesBeneficial
Large
Lactobacillus gasseri BNR17Increased Parasympathetic Nerve ActivityBeneficial
Moderate
Lactobacillus gasseri BNR17Reduced Clostridium Cluster IV ContentBeneficial
Large
Lactobacillus gasseri BNR17Reduced Fecal OdorBeneficial
Moderate
Lactobacillus gasseri BNR17Reduced Fecal p-Cresol LevelsBeneficial
Large
Lactobacillus gasseri KS-13Improved Bowel Movement FrequencyBeneficial
Moderate
Lactobacillus gasseri KS-13Increased Bifidobacterium Levels in FecesBeneficial
Large
Lactobacillus gasseri KS-13Increased Parasympathetic Nervous System ActivityBeneficial
Moderate
Lactobacillus gasseri KS-13Reduced Clostridium Cluster IV ContentBeneficial
Large
Lactobacillus gasseri KS-13Reduced Fecal p-Cresol LevelsBeneficial
Large
Lactobacillus gasseri LAC-343Enhanced Autonomic Activity Total PowerBeneficial
Large
Lactobacillus gasseri LAC-343Improved Fecal OdorBeneficial
Moderate
Lactobacillus gasseri LAC-343Improved Parasympathetic ActivityBeneficial
Moderate
Lactobacillus gasseri LAC-343Increased Bifidobacterium Levels in FecesBeneficial
Large
Lactobacillus gasseri LAC-343Increased Bowel MovementsBeneficial
Moderate
Lactobacillus gasseri LAC-343Reduced Clostridium Cluster IV ContentBeneficial
Large
Lactobacillus gasseri LAC-343Reduced Fecal p-Cresol LevelsBeneficial
Large
Lactobacillus gasseri SD-5585Improved Stool FrequencyBeneficial
Moderate
Lactobacillus gasseri SD-5585Increased Bifidobacterium Levels in FecesBeneficial
Large
Lactobacillus gasseri SD-5585Increased Parasympathetic Nervous System ActivityBeneficial
Moderate
Lactobacillus gasseri SD-5585Reduced Clostridium Cluster IV ContentBeneficial
Large
Lactobacillus gasseri SD-5585Reduced Fecal OdorBeneficial
Moderate
Lactobacillus gasseri SD-5585Reduced Fecal p-Cresol LevelsBeneficial
Large
Lactobacillus gasseri UALg-05Enhanced Autonomic Nervous System ActivityBeneficial
Moderate
Lactobacillus gasseri UALg-05Improved Stool FrequencyBeneficial
Moderate
Lactobacillus gasseri UALg-05Increased Bifidobacterium Levels in FecesBeneficial
Large
Lactobacillus gasseri UALg-05Increased Parasympathetic Nerve ActivityBeneficial
Moderate
Lactobacillus gasseri UALg-05Reduced Clostridium Cluster IV ContentBeneficial
Large
Lactobacillus gasseri UALg-05Reduced Fecal OdorBeneficial
Moderate
Lactobacillus gasseri UALg-05Reduced Fecal p-Cresol LevelsBeneficial
Large
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