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Safety assessment of the human milk-isolated probiotic Lactobacillus salivarius CECT5713.

  • 2007-08
  • Journal of Dairy Science 90(8)
    • F. Lara‐Villoslada
    • S. Sierra
    • M. Díaz-Ropero
    • M. Olivares
    • J. Xaus

Abstract

The potential probiotic bacteria Lactobacillus salivarius CECT5713 has recently been isolated from human milk and characterized. The objective of the present study was to evaluate the oral toxicity of this potential probiotic bacteria in mice. With this aim, 50 Balb/C mice were divided in 5 groups (n = 10). Three of these groups were treated orally with different doses of L. salivarius CECT5713: 5 x 10(8), 2 x 10(9), or 10(10) cfu/mouse per d for 28 d. One additional group was administered the vehicle alone and was used as a control. The last group were injected intraperitoneally with 10(8) cfu/mouse in a single dose and killed 2 (n = 5) and 5 (n = 5) d after intraperitoneal injection. Food intake, body weight, bacterial translocation, serum alpha-amyloid protein, and different biochemical parameters were analyzed. Oral administration of L. salivarius CECT5713 to mice had no adverse effects on mouse body weight or food intake. No bacteremia was shown and there was no treatment-associated bacterial translocation to the liver or spleen. Intraperitoneal administration caused a significant bacterial translocation to the liver and spleen, but not to the blood. However, this translocation was not related to illness or death at either d 2 or d 5, although an increase in plasma serum alpha-amyloid protein was observed at d 2. These results suggest that the strain L. salivarius CECT5713 is nonpathogenic for mice, even in doses 10,000 times higher (expressed per kilograms of body weight) than those normally consumed by humans. Thus, this strain is likely to be safe for human consumption.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus SalivariusAbsence of BacteremiaNeutral
Small
Lactobacillus SalivariusIncreased Plasma Serum Alpha-Amyloid ProteinNeutral
Moderate
Lactobacillus SalivariusNo Adverse Effects on Body Weight or Food IntakeNeutral
Moderate
Lactobacillus SalivariusNo Illness or Death from Bacterial Translocation (Intraperitoneal Administration)Neutral
Large
Lactobacillus salivarius CRL 1328Ensured Safety for Human ConsumptionBeneficial
Moderate
Lactobacillus salivarius CRL 1328Increased Plasma Serum Alpha-Amyloid ProteinNeutral
Moderate
Lactobacillus salivarius CRL 1328No Adverse Weight or Intake Effects in MiceNeutral
Large
Lactobacillus salivarius CRL 1328No Bacteremia or Translocation of Bacteria to Vital OrgansNeutral
Small
Lactobacillus salivarius CRL 1328Nonpathogenic for MiceNeutral
Large
Lactobacillus salivarius HA-118Increased Plasma Serum Alpha-Amyloid ProteinNeutral
Moderate
Lactobacillus salivarius HA-118No Adverse Effects on Body Weight or Food IntakeNeutral
Moderate
Lactobacillus salivarius HA-118No BacteremiaNeutral
Moderate
Lactobacillus salivarius HA-118Nonpathogenic Even at High DosesNeutral
Large
Lactobacillus salivarius SD-5208Increased Plasma Serum Alpha-Amyloid ProteinNeutral
Moderate
Lactobacillus salivarius SD-5208No Adverse Effects on Body Weight or Food IntakeNeutral
Moderate
Lactobacillus salivarius SD-5208No Bacteremia or Translocation of Bacteria to Vital OrgansNeutral
Small
Lactobacillus salivarius SD-5851Increased Plasma Serum Alpha-Amyloid ProteinNeutral
Moderate
Lactobacillus salivarius SD-5851No Adverse Effects from Intraperitoneal AdministrationNeutral
Small
Lactobacillus salivarius SD-5851No Adverse Weight or Intake Effects in MiceNeutral
Large
Lactobacillus salivarius SD-5851No Bacteremia or Translocation of Bacteria to Vital OrgansNeutral
Small
Lactobacillus salivarius SD-5851Nonpathogenic in High Doses for MiceNeutral
Moderate
Lactobacillus salivarius UALb-07Absence of Bacterial Translocation to Liver or SpleenNeutral
Moderate
Lactobacillus salivarius UALb-07Increased Serum Amyloid ProteinHarmful
Large
Lactobacillus salivarius UALb-07No Adverse Effects on Body Weight or Food IntakeNeutral
Moderate
Lactobacillus salivarius UALs07Maintained Body WeightNeutral
Large
Lactobacillus salivarius UALs07Reduced Bacterial TranslocationBeneficial
Large
Lactobacillus salivarius UALs07 UASAbsence of BacteremiaNeutral
Small
Lactobacillus salivarius UALs07 UASEnsured Safety for Human ConsumptionBeneficial
Moderate
Lactobacillus salivarius UALs07 UASNo Hepatic or Splenic Translocation with Oral AdministrationNeutral
Small
Lactobacillus salivarius UALs07 UASNo Illness or Death Despite Bacterial TranslocationNeutral
Small
Lactobacillus salivarius UALs07 UASNonpathogenic ConditionNeutral
Small
Lactobacillus salivarius UCC118Absence of Treatment-Associated Bacterial TranslocationNeutral
Large
Lactobacillus salivarius UCC118Increased Plasma Serum Alpha-Amyloid ProteinNeutral
Moderate
Lactobacillus salivarius UCC118No Adverse Effects on Body Weight or Food IntakeNeutral
Moderate
Lactobacillus salivarius UCC118Nonpathogenic Even at High DosesNeutral
Large
Lactobacillus salivarius UCC118Significant Bacterial Translocation with Intraperitoneal AdministrationHarmful
Moderate
Lactobacillus salivarius VPro 15No Bacteremia or Translocation of Bacteria to Vital OrgansNeutral
Small
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