Screening and Characterization of Pediococcus acidilactici LC-9-1 toward Selection as a Potential Probiotic for Poultry with Antibacterial and Antioxidative Properties

2023-01-17
Antioxidants 12(2)
- Chong Li
- Shaolong Wang
- Si Chen
- Xiaoying Wang
- Xuejuan Deng
- Guohua Liu
- Wenhuan Chang
- Y. Beckers
- Huiyi Cai

PubMed: 36829774
DOI: 10.3390/antiox12020215

Study Design

Type: Clinical Trial
Population: Broiler chickens
Methods: Controlled experimental study

Animal Study

Abstract

Growing interest has been focused on lactic acid bacteria as alternatives to antimicrobial growth promoters, which are characterized by the production of various functional metabolites, such as antimicrobial and antioxidants compounds. The present study was undertaken to evaluate a potential probiotic from the antioxidant perspective. LC-9-1, screened from the intestines of healthy animals, was revealed to be Pediococcus acidilactici on the basis of its morphological, biochemical, and molecular characteristics. The strain has excellent properties, including acid-production efficiency, antibacterial performance and antioxidant activity. The safety of the strain was also evaluated. Furthermore, the experiments in broiler chickens suggested that dietary LC-9-1 supplementation improved the growth performance and decreased the abdominal fat, and enhanced the antioxidant capability and intestinal innate immunity of broilers. Analysis of intestinal microbiota showed that a higher community diversity (Shannon index) was achieved. In addition to the significantly increased relative abundances of Pediococcus spp., beneficial genera such as Rothia spp. and Ruminococcus spp. were abundant, while opportunistic pathogens such as Escherichia-Shigella spp. were significantly reduced in LC-9-1-supplemented broilers. Collectively, such in-depth characterization and the available data will guide future efforts to develop next-generation probiotics, and LC-9-1 could be considered a potential strain for further utilization in direct-fed microbial or starter culture for fermentation.

Keywords: Pediococcus acidilactici; antibacterial; antioxidant; broiler chickens; intestinal microbiota; screening.

Research Insights

Pediococcus acidilactici KABP-021→Improved Abundance of Beneficial Bacteria
beneficial genera such as *Rothia* spp. and *Ruminococcus* spp. were abundant
Effect
Beneficial
Effect size
Moderate
Pediococcus acidilactici KABP-021→Improved Antioxidant Function
and enhanced the antioxidant capability
Effect
Beneficial
Effect size
Moderate
Pediococcus acidilactici KABP-021→Improved Gut Microbiota Diversity
Analysis of intestinal microbiota showed that a higher community diversity (Shannon index) was achieved.
Effect
Beneficial
Effect size
Moderate
Pediococcus acidilactici KABP-021→Improved Intestinal Innate Immunity
and intestinal innate immunity of broilers
Effect
Beneficial
Effect size
Moderate
Pediococcus acidilactici KABP-021→Reduced Abdominal Fat
and decreased the abdominal fat
Effect
Beneficial
Effect size
Moderate
Pediococcus acidilactici KABP-021→Reduced Opportunistic Pathogens
while opportunistic pathogens such as Escherichia-Shigella spp. were significantly reduced in LC-9-1-supplemented broilers
Effect
Beneficial
Effect size
Moderate
Pediococcus acidilactici MAK92P26A→Improved Antioxidant Function
and enhanced the antioxidant capability
Effect
Beneficial
Effect size
Moderate
Pediococcus acidilactici MAK92P26A→Improved Gut Microbiota Diversity
Analysis of intestinal microbiota showed that a higher community diversity (Shannon index) was achieved.
Effect
Beneficial
Effect size
Moderate
Pediococcus acidilactici MAK92P26A→Improved Intestinal Innate Immunity
and intestinal innate immunity of broilers
Effect
Beneficial
Effect size
Moderate
Pediococcus acidilactici MAK92P26A→Increased Beneficial Gut Bacteria
In addition to the significantly increased relative abundances of Pediococcus spp., beneficial genera such as Rothia spp. and Ruminococcus spp. were abundant
Effect
Beneficial
Effect size
Moderate
Pediococcus acidilactici MAK92P26A→Reduced Abdominal Fat
and decreased the abdominal fat
Effect
Beneficial
Effect size
Moderate
Pediococcus acidilactici MAK92P26A→Reduced Opportunistic Pathogens
while opportunistic pathogens such as Escherichia-Shigella spp. were significantly reduced in LC-9-1-supplemented broilers
Effect
Beneficial
Effect size
Moderate
Pediococcus acidilactici VPro29→Improved Antioxidant Function
and enhanced the antioxidant capability
Effect
Beneficial
Effect size
Moderate
Pediococcus acidilactici VPro29→Improved Growth Performance
the experiments in broiler chickens suggested that dietary LC-9-1 supplementation improved the growth performance and decreased the abdominal fat
Effect
Beneficial
Effect size
Large
Pediococcus acidilactici VPro29→Improved Gut Microbiota Diversity
Analysis of intestinal microbiota showed that a higher community diversity (Shannon index) was achieved.
Effect
Beneficial
Effect size
Moderate
Pediococcus acidilactici VPro29→Improved Intestinal Innate Immunity
and intestinal innate immunity of broilers
Effect
Beneficial
Effect size
Moderate
Pediococcus acidilactici VPro29→Reduced Abdominal Fat
and decreased the abdominal fat
Effect
Beneficial
Effect size
Moderate
Pediococcus acidilactici VPro29→Reduced Pathogen Infection
while opportunistic pathogens such as Escherichia-Shigella spp. were significantly reduced in LC-9-1-supplemented broilers.
Effect
Beneficial
Effect size
Moderate