SIRT1 mediated autophagy enhancement by Lactobacillus fermentum derived oligosaccharides accelerates wound healing in biofilm associated infection.

2025-12-26
Scientific reports 15(1)

PubMed: 41453907
DOI: 10.1038/s41598-025-30280-2

Study Design

Population: In vivo wound-healing assays in rats
Methods: Structural elucidation of OligoF using paper chromatography and 1D & 2D NMR spectroscopy; antibacterial testing against multidrug-resistant Pseudomonas aeruginosa isolates with inhibition zones, MIC, and MBC; biofilm eradication assays and scanning electron microscopy; in vivo wound-healing assays in rats with OligoF treatment

Rigorous Journal

The present study explores the structural elucidation, antimicrobial properties, and wound-healing potential of a novel oligosaccharide, OligoF, derived from Lactobacillus fermentum. Structural analysis using paper chromatography, and 1D & 2D NMR spectroscopy indicated that OligoF is a linear tetrasaccharide comprising β-D-glucose, α-L-rhamnose, and D-glucuronic acid in a defined sequence. This structural elucidation was further confirmed by key chemical shifts, coupling constants, and glycosidic linkage assignments. OligoF exhibited potent antibacterial activity against multidrug-resistant Pseudomonas aeruginosa isolates, demonstrating inhibition zones of 60-63 mm, a minimum inhibitory concentration (MIC) of 16 µg/mL, and a minimum bactericidal concentration (MBC) of 32 µg/mL. Biofilm eradication assays revealed a significant reduction in biofilm viability by ~ four-fold at sub-MIC concentrations, as evidenced by scanning electron microscopy. OligoF-treated biofilms displayed notable disruption of extracellular matrix and severe bacterial cell distortion compared to untreated controls. In vivo wound-healing assays in rats showed that OligoF significantly reduced wound area and enhanced skin repair compared to untreated controls. Treatment with OligoF notably increased the concentration and gene expression of SIRT1 as well as upregulated the gene expression of beclin1 and ATG5 which are critical regulators of autophagy and cellular repair processes. Histopathological analysis corroborated these findings, revealing enhanced re-epithelialization, granulation tissue formation, and vascularized connective tissue deposition in OligoF-treated wounds. These findings underscore the potential of OligoF as a multifunctional agent with antibacterial and wound-healing properties, paving the way for its application in managing multidrug-resistant infections and promoting tissue repair.

Research Insights

Supplement	Dose	Health Outcome	Effect Type	Effect Size	Source
Lactobacillus fermentum	—	Improved Wound Healing	Beneficial	Large	View source OligoF significantly reduced wound area and enhanced skin repair compared to untreated controls.
Lactobacillus fermentum	—	Reduced Bacterial Load	Beneficial	Large	View source OligoF exhibited potent antibacterial activity against multidrug-resistant Pseudomonas aeruginosa isolates, demonstrating inhibition zones of 60-63 mm, a minimum inhibitory concentration (MIC) of 16 µg/mL, and a minimum bactericidal concentration (MBC) of 32 µg/mL.
Lactobacillus fermentum	—	Reduced Biofilm Mass	Beneficial	Large	View source Biofilm eradication assays revealed a significant reduction in biofilm viability by ~ four-fold at sub-MIC concentrations