Streptococcus thermophilus extends lifespan through activation of DAF-16-mediated antioxidant pathway in Caenorhabditis elegans
- 2022
- Journal of Clinical Biochemistry and Nutrition 70(1)
- Natsumi Desaka
- Chinatsu Ota
- Hitomi Nishikawa
- K. Yasuda
- N. Ishii
- T. Bito
- Yukio Kishinaga
- Y. Naito
- Yasuki Higashimura
- PubMed: 35068675
- DOI: 10.3164/jcbn.21-56
Abstract
Streptococcus thermophilus bacteria, which are widely used as fermented starter for dairy production, exert various beneficial health effects. Nevertheless, even though pro-longevity effects of various probiotics have been reported, no report has described Streptococcus thermophilus effects on longevity. This study was conducted to evaluate Streptococcus thermophilus effects on lifespan extension and to elucidate the Streptococcus thermophilus-mediated longevity mechanism using Caenorhabditis elegans worms as a model animal. They were fed standard food (Escherichia coli OP50) or Streptococcus thermophilus from the young adult stage. Feeding with Streptococcus thermophilus, compared to Escherichia coli OP50, to Caenorhabditis elegans extend the lifespan, reduced lipofuscin accumulation, and maintain vigorous locomotion. Feeding with Streptococcus thermophilus did not alter the worm growth curve or the offspring number, indicating that the Streptococcus thermophilus-mediated lifespan extension is not attributable to caloric restriction. The qRT-PCR data showed that Streptococcus thermophilus increased the expression of daf-16 and some of its downstream antioxidant genes. Furthermore, the pro-longevity effects of Streptococcus thermophilus were decreased in loss-of-function mutant of daf-16. Results show that Streptococcus thermophilus extends the lifespan of Caenorhabditis elegans through DAF-16-mediated antioxidant pathway activation.
Keywords: Caenorhabditis elegans; hydrogen peroxide; lactic acid bacteria; longevity; oxidative stress.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Streptococcus thermophilus | Extended Lifespan | Beneficial | Moderate |
| Streptococcus thermophilus | Maintained Vigorous Locomotion | Beneficial | Moderate |
| Streptococcus thermophilus | Reduced Lipofuscin Accumulation | Beneficial | Moderate |
| Streptococcus thermophilus MAK34S24T | Extended Lifespan | Beneficial | Moderate |
| Streptococcus thermophilus MAK34S24T | Increased Antioxidant Gene Expression | Beneficial | Moderate |
| Streptococcus thermophilus MAK34S24T | Maintained Vigorous Locomotion | Beneficial | Moderate |
| Streptococcus thermophilus NBRC 13957 | Extended Lifespan | Beneficial | Moderate |
| Streptococcus thermophilus NBRC 13957 | Maintained Vigorous Locomotion | Beneficial | Moderate |
| Streptococcus thermophilus NBRC 13957 | Reduced Lipofuscin Accumulation | Beneficial | Moderate |
| Streptococcus thermophilus PXN 66 | Increased Antioxidant Activity | Beneficial | Large |
| Streptococcus thermophilus PXN 66 | Increased Lifespan | Beneficial | Moderate |
| Streptococcus thermophilus PXN 66 | Maintained Vigorous Locomotion | Beneficial | Moderate |
| Streptococcus thermophilus PXN 66 | Reduced Lipofuscin Accumulation | Beneficial | Moderate |
| Streptococcus thermophilus SEBiotic | Increased Lifespan | Beneficial | Moderate |
| Streptococcus thermophilus SEBiotic | Maintained Physical Activity Level | Beneficial | Moderate |
| Streptococcus thermophilus SEBiotic | Reduced Hepatic Oxidative Stress | Beneficial | Moderate |
| Streptococcus thermophilus SL-21 | Increased Lifespan | Beneficial | Moderate |
| Streptococcus thermophilus SL-21 | Maintained Vigorous Locomotion | Beneficial | Moderate |
| Streptococcus thermophilus SL-21 | Reduced Lipofuscin Accumulation | Beneficial | Moderate |
| Streptococcus thermophilus SP4 | Enhanced Antioxidant Gene Expression | Beneficial | Moderate |
| Streptococcus thermophilus SP4 | Increased Lifespan | Beneficial | Moderate |
| Streptococcus thermophilus SP4 | Maintained Growth Curve | Neutral | Small |
| Streptococcus thermophilus SP4 | Maintained Vigorous Locomotion | Beneficial | Moderate |
| Streptococcus thermophilus SP4 | Reduced Lipofuscin Accumulation | Beneficial | Moderate |
| Streptococcus thermophilus SP4 | Unchanged Offspring Number | Neutral | Small |
| Streptococcus thermophilus ST-21 | Increased Antioxidant Activity | Beneficial | Large |
| Streptococcus thermophilus ST-21 | Increased Lifespan | Beneficial | Moderate |
| Streptococcus thermophilus ST-21 | Maintained Vigorous Locomotion | Beneficial | Moderate |
| Streptococcus thermophilus ST-21 | Reduced Lipofuscin Accumulation | Beneficial | Moderate |
| Streptococcus thermophilus ST1447 | Increased Lifespan | Beneficial | Moderate |
| Streptococcus thermophilus ST1447 | Maintained Vigorous Locomotion | Beneficial | Moderate |
| Streptococcus thermophilus ST1447 | Reduced Lipofuscin Accumulation | Beneficial | Moderate |
| Streptococcus thermophilus TH-4 | Increased Lifespan | Beneficial | Moderate |
| Streptococcus thermophilus TH-4 | Maintained Vigorous Locomotion | Beneficial | Moderate |
| Streptococcus thermophilus TH-4 | Reduced Lipofuscin Accumulation | Beneficial | Moderate |
| Streptococcus thermophilus UASt-09 | Increased Lifespan | Beneficial | Moderate |
| Streptococcus thermophilus UASt-09 | Maintained Vigorous Locomotion | Beneficial | Moderate |
| Streptococcus thermophilus UASt-09 | Reduced Lipofuscin Accumulation | Beneficial | Moderate |
| Streptococcus thermophilus VPro 23 | Increased Lifespan | Beneficial | Moderate |
| Streptococcus thermophilus VPro 23 | Maintained Vigorous Locomotion | Beneficial | Moderate |
| Streptococcus thermophilus VPro 23 | Reduced Lipofuscin Accumulation | Beneficial | Moderate |
| Streptococcus thermophilus Y08 | Increased Lifespan | Beneficial | Moderate |
| Streptococcus thermophilus Y08 | Maintained Vigorous Locomotion | Beneficial | Moderate |
| Streptococcus thermophilus Y08 | Reduced Lipofuscin Accumulation | Beneficial | Moderate |
| Streptococcus thermophilus Y08 AF | Extended Lifespan | Beneficial | Moderate |
| Streptococcus thermophilus Y08 AF | Increased Antioxidant Gene Expression | Beneficial | Moderate |
| Streptococcus thermophilus Y08 AF | Maintained Vigorous Locomotion | Beneficial | Moderate |
| Streptococcus thermophilus Y08 AF | Reduced Lipofuscin Accumulation | Beneficial | Moderate |