V9 on dextran sodium sulfate-induced colitis in mice.

2025-01-24
Frontiers in medicine 12
- Xiaoyan Duan
- Rilige Wu
- Jianbo Li
- Zeya Li
- Yanqi Liu
- Ping Chen
- Bangmao Wang

PubMed: 39926427
DOI: 10.3389/fmed.2025.1496023

Background

Inflammatory bowel disease (IBD) has become a global public health problem with complex pathogenesis and limited therapeutic options. We aimed to investigate the potential mechanisms by which Bifidobacterium lactis V9 (V9) alleviated colitis in a dextran sodium sulfate-induced colitis model mice.

Methods

Mice were induced to develop colitis by drinking DSS solution to induce colitis. The expression of the relevant factors in the blood supernatant of the mice was determined by ELISA. RT-qPCR and Western blotting were used to detect mRNA and protein expression of target genes. The fecal microbiota was analyzed by 16S rRNA sequencing. Intestinal metabolites were analyzed by untargeted metabolomics.

Results

V9 effectively improved the overall symptoms of the colitis model mice. H&E showed that V9 re-stored the intestinal tissue structure. ELISA showed that V9 decreased the levels of IL-6, IL-22, and TNF-α and increased IL-10, SP, VIP, and 5-HT. V9 increased the expression of AHR, CYP1A1, MUC2, Claudin-3, Occludin, and ZO-1, and decreased 5-hydroxytryptamine transporter and Claudin-2. V9 increased the abundance of gut microbiota in colitis mice to promote the growth of beneficial bacteria. V9 increased tryptophan metabolites, and short-chain fatty acids, and improved gut inflammation.

Conclusion

V9 attenuates intestinal inflammation, improves the mucosal barrier, modulates intestinal microecology and exerts a protective effect in a mouse model of DSS-induced colitis.

Research Insights

Supplement	Health Outcome	Effect Type	Effect Size
Bifidobacterium lactis	Altered Gut Microbiota Composition	Beneficial	Moderate
Bifidobacterium lactis	Improved Intestinal Barrier Function	Beneficial	Large
Bifidobacterium lactis	Increased Levels of Beneficial Metabolites	Beneficial	Moderate
Bifidobacterium lactis	Reduced Colitis Severity	Beneficial	Large
Bifidobacterium lactis	Reduced Intestinal Inflammation	Beneficial	Large