Synergy between Lactobacillus paracasei and its bacterial products to counteract stress-induced gut permeability and sensitivity increase in rats.
- 2007-08
- The Journal of Nutrition 137(8)
- H. Eutamene
- Florence Lamine
- C. Chabo
- V. Théodorou
- F. Rochat
- G. Bergonzelli
- I. Corthésy-theulaz
- J. Fioramonti
- L. Buéno
- PubMed: 17634262
- DOI: 10.1093/JN/137.8.1901
Abstract
Stressful events result in the alteration of gut permeability and sensitivity. Lactobacillus paracasei NCC2461 (Lpa) therapy prevents antibiotic-induced visceral hyperalgesia in mice. This study aimed at evaluating the influence of 3 probiotic strains: Bifidobacterium lactis NCC362, Lactobacillus johnsonii NCC533, and Lpa on stress-mediated alterations of colorectal hyperalgesia, on gut paracellular permeability and whether bacteria and/or bacterial products present in the spent culture medium (SCM) were involved in the antinociceptive properties of the effective strain. Rat pups were separated from their mothers 3 h/d during postnatal d 2-14. At wk 13, gut paracellular permeability was determined as a percentage of urinary excreted (51)Cr-EDTA and visceral sensitivity to colorectal distension (CRD), assessed by abdominal muscle electromyography. Visceral sensitivity was also analyzed in adults rats subjected to partial restraint stress (PRS, 2 h restriction of body movements). Rats received either the probiotics resuspended in SCM or fresh growth medium as control for 2 wk. Maternal deprivation significantly increased colonic sensitivity in response to CRD and enhanced gut paracellular permeability compared with control rats. Only Lpa treatment significantly improved stress-induced visceral pain and restored normal gut permeability. Similarly, among the 3 probiotics tested, only Lpa prevented PRS-mediated visceral hyperalgesia. Both bacteria and bacterial products present in Lpa SCM were required for the antinociceptive properties against PRS. This study illustrates strain-specific effects and suggests a synergistic interplay between L. paracasei bacteria and bacterial products generated during fermentation and growth that confers the ability to suppress PRS-induced hypersensitivity in rats.
Research Insights
Supplement | Health Outcome | Effect Type | Effect Size |
---|---|---|---|
Lacticaseibacillus paracasei Lpc-37 | Improved Gut Permeability | Beneficial | Moderate |
Lacticaseibacillus paracasei Lpc-37 | Prevented Visceral Hyperalgesia | Beneficial | Moderate |
Lacticaseibacillus paracasei Lpc-37 | Reduced Stress-Mediated Visceral Hyperalgesia | Beneficial | Large |
Lactobacillus paracasei LPC09 | Improved Epithelial Permeability | Beneficial | Large |
Lactobacillus paracasei LPC09 | Reduced Stress-Mediated Visceral Hyperalgesia | Beneficial | Large |
Lactobacillus paracasei LPC09 | Reduced Visceral Pain | Beneficial | Large |