Systematic Analysis Reveals Two Novel O-Methyltransferase Genes Involved in Flavonoid Biosynthesis in Ginkgo Leaves.
- 2026-03-25
- Journal of agricultural and food chemistry 74(13)
- Manman Shi
- Xiaoming Yang
- Guibin Wang
- Fuliang Cao
- Yingying Sun
- Erzheng Su
- PubMed: 41881800
- DOI: 10.1021/acs.jafc.5c17265
Study Design
- Methods
- Transcriptomic analysis of ginkgo leaves at different developmental stages identified two FOMTs; enzymatic assays with seven flavonoid substrates tested methylation activity.
- Funding
- Unclear
- Rigorous Journal
Flavonoids are widely distributed in plants, including Ginkgo biloba, a traditional medicinal and edible species. Methylated flavonoids often exhibit enhanced biological activities compared with their unmethylated forms, and flavonoid O-methyltransferases (FOMTs) responsible for this modification have been identified in several plants but remain poorly characterized in ginkgo. In this study, transcriptomic analysis of ginkgo leaves at different developmental stages identified two S-adenosylmethionine-dependent FOMTs, GbFOMT3 and GbFOMT12. Enzymatic assays with seven flavonoid substrates showed that both enzymes methylate quercetin, eriodictyol, and luteolin to produce isorhamnetin, hesperetin, and chrysoeriol, respectively, through methylation at the 3'-OH and 4'-OH positions of the B ring. These findings expand the repertoire of ginkgo flavonoid O-methyltransferases and provide insights into flavonoid methylation in ginkgo.