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Evidence-Based Supplement Research
Evidence-Based Supplement Research

Taoren Honghua Jian Regulate NLRP3 Inflammasome of Coronary Artery Disease Patients: A Multi-Center, Double-Blind, Randomized Controlled Trial.

  • 2026-04-14
  • Vascular health and risk management 22
    • Min Du
    • Xiaoteng Feng
    • Na Zhang
    • Meijiao Mao
    • Leyi Du
    • Ying Yang
    • Yifan Zhang
    • Sijin Li
    • Jiarou Wang
    • Xindi Chang
    • Jie Ding
    • Ping Liu
    • Yiyi Zhang
    • Yiru Wang

Study Design

Type
Randomized Controlled Trial (RCT)
Sample size
n = 120
Population
120 eligible CAD patients from three Shanghai hospitals
Methods
multicenter, double-blind, randomized controlled trial; THJ granule (18.3 g, twice daily) or placebo for four weeks, with a four-week follow-up
Blinding
Double-blind
Duration
four weeks
  • Large Human Trial

Objective

This multicenter, double-blind, randomized controlled trial sought to assess the clinical efficacy of Taoren Honghua Jian (THJ) in patients with coronary artery disease (CAD) exhibiting Qi stagnation and blood stasis syndrome, and investigated its effect on NLRP3 inflammasome expression in peripheral blood mononuclear cells (PBMCs).

Methods

One hundred and twenty eligible CAD patients from three Shanghai hospitals were randomized to receive either the THJ granule (18.3 g, twice daily) or placebo for four weeks, with a four-week follow-up. Traditional Chinese Medicine Syndrome Score (TCMSS), Seattle Angina Questionnaire (SAQ), and lipid levels were measured before and after treatments. NLRP3 inflammasome components were examined in PBMCs using quantitative PCR, whereas plasma inflammatory cytokines were detected using ELISA.

Results

A total of 120 participants participated in the trial. The THJ group showed reduced TCMSS compared to the placebo group (P < 0.01). After four weeks of intervention, the THJ group scored considerably higher on five SAQ aspects compared to the placebo group (P < 0.01). However, lipid levels showed no significance. In PBMCs, THJ lowered mRNA expression of NLRP3 inflammasome components (NLRP3, ASC, caspase-1, IL-1β, IL-18) (P < 0.01). Patients in the THJ group showed significantly lower plasma levels of IL-1β, IL-2, and IL-18 following therapy compared to the placebo group (P < 0.01).

Conclusion

THJ reduces angina symptoms and improves quality of life in CAD patients, which suggests that it suppresses NLRP3-related transcriptional activity and hence reduces pro-inflammatory cytokine production. These data suggest that THJ might be an effective adjuvant treatment for inflammation-driven coronary atherosclerosis.

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