The Ability of Streptococcus thermophilus BT01 to Modulate Urease Activity in Healthy Subjects' Fecal Samples Depends on the Biomass Production Process.
- 2023-02-14
- Molecular Nutrition & Food Research 67(6)
- Martinović Anđela
- Chittaro Marco
- Mora Diego
- Arioli Stefania
- PubMed: 36708131
- DOI: 10.1002/mnfr.202200529
Abstract
Scope: This study evaluates how manufacturing conditions of probiotic biomass production, using two different cryoprotectants, Cryo-A and Cryo-B, can affect Streptococcus thermophilus BT01 in vivo gastrointestinal tract survival and its ability to modulate the level of urease activity in fecal samples of healthy subjects.
Methods and results: A randomized controlled cross-over study is carried out on 20 adult healthy subjects to evaluate total and viable loads, persistence of S. thermophilus BT01, and urease activity in fecal samples. Strain-specific quantification by using developed culture-based method and molecular qPCR tool allows to quantify viable S. thermophilus BT01 strain in 90% of the subjects. The quantification of both total DNA and recovered viable S. thermophilus BT01 in fecal samples does not reveal significant differences between Cryo-A or Cryo-B treated biomass. However, the administration of S. thermophilus BT01 produced with Cryo-A results in a decreased urease activity in fecal samples compared to Cryo-B protected cells.
Conclusion: This study i) highlights how the manufacturing conditions can play a role in influencing the probiotic functionality in vivo and ii) represents the first evidence that links S. thermophilus to a specific probiotic mechanism, the reduction of urease activity in fecal samples.
Keywords: Streptococcus thermophilus; cryoprotectant; interventional study; probiotic; urease.
Research Insights
Supplement | Health Outcome | Effect Type | Effect Size |
---|---|---|---|
Streptococcus thermophilus SEBiotic | Reduced Urease Activity | Beneficial | Moderate |