The Effects of Bacillus subtilis QST713 and β-mannanase on growth performance, intestinal barrier function, and the gut microbiota in weaned piglets.
- 2023-01-01
- Journal of animal science 101
- Junchen Liu
- Xiangyuan Ma
- Yong Zhuo
- Shengyu Xu
- Lun Hua
- Jian Li
- Bin Feng
- Zhengfeng Fang
- Xuemei Jiang
- Lianqiang Che
- Zeyuan Zhu
- Yan Lin
- De Wu
- De Wu
- PubMed: 37583344
- DOI: 10.1093/jas/skad257
Study Design
- Type
- Randomized Controlled Trial (RCT)
- Population
- 320 healthy piglets
- Methods
- randomly assigned to four groups: control group (basal diet), BS100 group (basal diet plus 100 mg/kg B. subtilis QST713), BS200 group (basal diet plus 200 mg/kg B. subtilis QST713), and BS100XT group (basal diet plus 100 mg/kg B. subtilis QST713 and 150 mg/kg β-mannanase)
- Duration
- 42 d
- Animal Study
We investigated the effects of different Bacillus subtilis QST713 doses and a B. subtilis QST713 and β-mannanase mix on growth performance, intestinal barrier function, and gut microbiota in weaned piglets. In total, 320 healthy piglets were randomly assigned to four groups: 1) control group (basal diet), 2) BS100 group (basal diet plus 100 mg/kg B. subtilis QST713), 3) BS200 group (basal diet plus 200 mg/kg B. subtilis QST713), and 4) a BS100XT group (basal diet plus 100 mg/kg B. subtilis QST713 and 150 mg/kg β-mannanase). The study duration was 42 d. We showed that feed intake in weaned piglets on days 1 to 21 was increased in group BS100 (P < 0.05), and that the feed conversion ratio in group BS100XT animals decreased throughout the study (P < 0.05). In terms of microbial counts, the BS100XT group showed reduced Escherichia coli and Clostridium perfringens numbers on day 21 (P < 0.05). Moreover, no significant α-diversity differences were observed across all groups during the study (P > 0.05). However, principal coordinates analysis indicated clear separations in bacterial community structures across groups (analysis of similarities: P < 0.05) on days 21 and 42. Additionally, E-cadherin, occludin, and zonula occludens-1 (ZO-1) expression in piglet feces increased (P < 0.05) by adding B. subtilis QST713 and β-mannanase to diets. Notably, this addition decreased short-chain fatty acid concentrations. In conclusion, B. subtilis QST713 addition or combined B. subtilis QST713 plus β-mannanase effectively improved growth performance, intestinal barrier function, and microbial balance in weaned piglets.