The impact of Lacticaseibacillus paracasei GMNL-143 toothpaste on gingivitis and oral microbiota in adults: a randomized, double-blind, crossover, placebo-controlled trial.

2024-04-20
BMC oral health 24(1)
- Min-Kang Lee
- I-Hui Chen
- I-Ling Hsu
- Wan-Hua Tsai
- Tzong-Yi Lee
- Jhih-Hua Jhong
- Bai-Chia Liu
- Tsui-Yin Huang
- Fang-Kuei Lin
- Wen-Wei Chang
- Ju-Hui Wu

PubMed: 38643116
DOI: 10.1186/s12903-024-04251-4

Study Design

Type: Randomized Controlled Trial (RCT)
Population: gingivitis patients
Methods: randomized, double-blind, crossover clinical trial using GMNL-143 toothpaste or placebo for four weeks, followed by a crossover after a washout
Blinding: Double-blind
Duration: four weeks
Funding: Unclear

Background

This study examines the oral health benefits of heat-killed Lacticaseibacillus paracasei GMNL-143, particularly its potential in oral microbiota alterations and gingivitis improvement.

Methods

We assessed GMNL-143's in vitro interactions with oral pathogens and its ability to prevent pathogen adherence to gingival cells. A randomized, double-blind, crossover clinical trial was performed on gingivitis patients using GMNL-143 toothpaste or placebo for four weeks, followed by a crossover after a washout.

Results

GMNL-143 showed coaggregation with oral pathogens in vitro, linked to its surface layer protein. In patients, GMNL-143 toothpaste lowered the gingival index and reduced Streptococcus mutans in crevicular fluid. A positive relationship was found between Aggregatibacter actinomycetemcomitans and gingival index changes, and a negative one between Campylobacter and gingival index changes in plaque.

Conclusion

GMNL-143 toothpaste may shift oral bacterial composition towards a healthier state, suggesting its potential in managing mild to moderate gingivitis.

Trial registration

ID NCT04190485 ( https://clinicaltrials.gov/ ); 09/12/2019, retrospective registration.