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The mortality of Bombyx mori larvae challenged by BmNPV is reduced when supplemented with Lactobacillus acidophilus bacteria.

  • 2024-12-17
  • BMC research notes 17(1)

Study Design

Type
Clinical Trial
Population
Thai polyvoltine strain Samrong silkworm larvae
Methods
silkworm larvae of the Thai polyvoltine strain Samrong was supplemented with commercial probiotic bacteria, Lactobacillus acidophilus, on the second day of the 2nd, 3rd, 4th, and 5th instar; challenged with BmNPV on the second day of the 4th instar
  • Animal Study

Objective

Bombyx mori nucleopolyhedrovirus (BmNPV) causes grasserie with severe effects in Thai strains of the silkworm Bombyx mori. The aim of this study was to investigate the effect of probiotic supplementation on the survival of silkworm larvae challenged with BmNPV.

Results

Silkworm larvae of the Thai polyvoltine strain Samrong was supplemented with commercial probiotic bacteria, Lactobacillus acidophilus, on the second day of the 2nd, 3rd, 4th, and 5th instar. When challenged with BmNPV on the second day of the 4th instar, the survival ratio was 92% for larvae supplemented with L. acidophilus as compared to 56% for larvae without L. acidophilus supplementation. For the larvae that survived until pupation, we determined the growth characters cocooning ratio, larval weight, and pupation ratio, and the economic characters cocoon shell weight and cocoon weight. Growth characters were significantly lower in larvae infected with BmNPV as compared to infected larvae receiving probiotics.

Research Insights

  • Lactobacillus acidophilus L-92→Improved Growth and Economic Silk Production Measures

    Growth characters were significantly lower in larvae infected with BmNPV as compared to infected larvae receiving probiotics.

    Effect
    Beneficial
    Effect size
    Moderate
  • Lactobacillus acidophilus L-92→Improved Growth and Economic Traits

    For the larvae that survived until pupation, we determined the growth characters cocooning ratio, larval weight, and pupation ratio, and the economic characters cocoon shell weight and cocoon weight. Growth characters were significantly lower in larvae infected with BmNPV as compared to infected larvae receiving probiotics.

    Effect
    Beneficial
    Effect size
    Moderate
  • Lactobacillus acidophilus L-92→Improved Survival

    When challenged with BmNPV on the second day of the 4th instar, the survival ratio was 92% for larvae supplemented with L. acidophilus as compared to 56% for larvae without L. acidophilus supplementation.

    Effect
    Beneficial
    Effect size
    Large
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