The mortality of Bombyx mori larvae challenged by BmNPV is reduced when supplemented with Lactobacillus acidophilus bacteria.
- 2024-12-17
- BMC research notes 17(1)
- Siripuk Suraporn
- Vallaya Suthikhum
- Olle Terenius
- PubMed: 39690411
- DOI: 10.1186/s13104-024-07019-9
Objective
Bombyx mori nucleopolyhedrovirus (BmNPV) causes grasserie with severe effects in Thai strains of the silkworm Bombyx mori. The aim of this study was to investigate the effect of probiotic supplementation on the survival of silkworm larvae challenged with BmNPV.Results
Silkworm larvae of the Thai polyvoltine strain Samrong was supplemented with commercial probiotic bacteria, Lactobacillus acidophilus, on the second day of the 2nd, 3rd, 4th, and 5th instar. When challenged with BmNPV on the second day of the 4th instar, the survival ratio was 92% for larvae supplemented with L. acidophilus as compared to 56% for larvae without L. acidophilus supplementation. For the larvae that survived until pupation, we determined the growth characters cocooning ratio, larval weight, and pupation ratio, and the economic characters cocoon shell weight and cocoon weight. Growth characters were significantly lower in larvae infected with BmNPV as compared to infected larvae receiving probiotics.Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus acidophilus L-92 | Improved Growth and Economic Silk Production Measures | Beneficial | Moderate |
| Lactobacillus acidophilus L-92 | Improved Growth and Economic Traits | Beneficial | Moderate |
| Lactobacillus acidophilus L-92 | Improved Survival | Beneficial | Large |