The Possible Role of Bifidobacterium longum BB536 and Lactobacillus rhamnosus HN001 on Locomotor Activity and Oxidative Stress in a Rotenone-Induced Zebrafish Model of Parkinson's Disease.

2021-01
Oxidative medicine and cellular longevity 2021(1)

PubMed: 34691361
DOI: 10.1155/2021/9629102

Study Design

Population: zebrafish (Danio rerio)
Methods: chronic administration of rotenone for 3 weeks

Background

As every organ within the body, the brain is also extremely susceptible to a plethora of noxious agents that change its chemistry. One component frequently found in current products against harmful species to crops is rotenone whose effect under prolonged exposure has been demonstrated to cause neurodegenerative disorders such as Parkinson's disease. The latest reports have indeed revealed that rotenone promotes Parkinson's in humans, but studies aiming to show congruent effects in zebrafish (Danio rerio) are lacking. Material and Methods. In this context, the aim of the present study was to demonstrate how chronic administration of rotenone for 3 weeks impairs the locomotor activity and sociability and induces oxidative stress in zebrafish.

Results

There were no statistically significant differences following the analysis of their social interaction and locomotor tests (p > 0.05). However, several exceptions have been noted in the control, rotenone, and probiotics groups when we compared their locomotor activity during the pretreatment and treatment interval (p < 0.05). We further assessed the role of rotenone in disturbing the detoxifying system as represented by three enzymes known as superoxide dismutase (SOD), glutathione peroxidase (GPx), and malondialdehyde (MDA). Despite the fact that there were no statistically significant changes within SOD and GPx levels between the control group and rotenone, probiotics, and rotenone + probiotics (p > 0.05), relevant changes have been observed between the analyzed groups (p < 0.05 and p < 0.005, respectively). On the other hand, significant differences (p < 0.05) have been observed for MDA when we analyzed the data between the control group and the other three groups.

Conclusions

Our results suggest that rotenone can be successfully used to trigger Parkinson's disease-related symptomatology in zebrafish.

Research Insights

Supplement	Dose	Health Outcome	Effect Type	Effect Size	Source
Lactobacillus rhamnosus HN001	—	No Change in Oxidative Stress Markers	Neutral	Small	View source there were no statistically significant changes within SOD and GPx levels between the control group and rotenone, probiotics, and rotenone + probiotics (p > 0.05)
Lactobacillus rhamnosus HN001	—	No Significant Improvement in Locomotor Activity	Neutral	Small	View source There were no statistically significant differences following the analysis of their social interaction and locomotor tests (p > 0.05).
Lactobacillus rhamnosus HN001	—	Reduced Lipid Peroxidation	Neutral	Small	View source significant differences (p < 0.05) have been observed for MDA when we analyzed the data between the control group and the other three groups.