Unexpected effect of probiotics by Lactococcus lactis subsp. lactis against colitis induced by dextran sulfate sodium in mice.
- 2020-06
- Journal of Infection and Chemotherapy 26(6)
- Shinichirou Komaki
- A. Haque
- H. Miyazaki
- Tetsuya Matsumoto
- Shigeki Nakamura
- PubMed: 32122783
- DOI: 10.1016/j.jiac.2020.01.006
Abstract
Ulcerative colitis (UC) is a representative intestinal chronic inflammatory disease whose incidence is rapidly increasing worldwide. It was previously shown that some specific probiotics help to guard against UC. In this study, we analyzed the effect of Lactococcus lactis subsp. lactis JCM5805 (L. lactis), which has been put to practical use as a probiotic, on the pathogenesis of UC using a dextran sulfate sodium-induced colitis mouse model. Survival rate, length, and histopathological parameters of the colon were elucidated. Further, the concentrations of inflammatory cytokines in serum were measured. As a result, the oral administration of high-dose L. lactis showed significant decreases in survival rate and colon length. Histopathological analysis showed that a bleeding appearance was observed in the L. lactis group, and the histology scores in the L. lactis group were significantly higher than those in the normal saline group. Furthermore, the levels of interferon gamma, tumor necrosis factor alpha, and interleukin-6 were significantly elevated in the L. lactis group. These results support that high-dose administration of L. lactis deteriorates intestinal inflammation and suggest that the careful selection of probiotics strains and administration dose is important for improving colitis including UC.
Keywords: Dextran sulfate sodium-induced colitis; Lactococcus lactis; Probiotics.
Research Insights
Supplement | Health Outcome | Effect Type | Effect Size |
---|---|---|---|
Lactococcus lactis subsp. lactis R1058 | Increased Colon Inflammation | Harmful | Large |
Lactococcus lactis subsp. lactis R1058 | Increased Inflammatory Cytokines | Harmful | Large |
Lactococcus lactis subsp. lactis R1058 | Reduced Survival Rate | Harmful | Large |