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Evidence-Based Supplement Research

Evidence-Based Supplement Research

Vitamin D and Increased 24,25-dihydroxyvitamin D Level

Doses used in studies

IU single-dose: 25,000–500,000 (median 262,500, IQR 143,750–381,250) — 2 studies

Safety in these studies

Overall tolerabilityReported
High-dose cholecalciferol supplementation was well tolerated
from: Effects of High-Dose Vitamin D Supplementation and Physical Exercise on Vitamin D Metaboli

2 of 2 papersPopulation:

Effects of High-Dose Vitamin D Supplementation and Physical Exercise on Vitamin D Metabolites in Professional Football Players: A Pilot Study.
- 2026-01-05
- RCT
- Nutrients 18(1)
- - Anna Książek
  - Aleksandra Zagrodna
  - Konrad Kowalski
- PubMed: 41515291
- DOI: 10.3390/nu18010175
3 Insights on
- Increased 24,25-dihydroxyvitamin D Level
- High evidence
- Rigorous Journal
- Athletes
Physical exercise during the football match significantly increased serum concentrations of ... 24,25-(OH)2D3 ... Following supplementation, these effects were further amplified
Effect
Beneficial
Effect size
Small
Significant
Yes
Dose
500,000 IU single dose
Short-Term Effects of Escalating Doses of Cholecalciferol on FGF23 and 24,25(OH)2 Vitamin D Levels: A Preliminary Investigation.
- 2024-10-23
- RCT
- Nutrients 16(21)
- - Jessica Pepe
  - Luciano Colangelo
  - Roberta Pilotto
  - Viviana De Martino
  - Carla Ferrara
  - Alfredo Scillitani
  - Mirella Cilli
  - Salvatore Minisola
  - Ravinder Singh
  - Cristiana Cipriani
- PubMed: 39519433
- DOI: 10.3390/nu16213600
10 Insights on
- Increased 24,25-dihydroxyvitamin D Level
- High evidence
- Rigorous Journal
- Clinical
In the group administered 600,000 I.U., there was a significant increase in the delta 24,25(OH)D2 compared to the placebo and 25,000 I.U. groups
Effect
Beneficial
Effect size
Small
Significant
Yes
Dose
600,000 I.U. single oral dose