Lactobacillus rhamnosus and Improved Intestinal Barrier Function

Research synthesisLow evidenceModerate effect4 studies · 4 beneficial · 0 neutral · 0 harmful

Across 4 studies, all reported beneficial moderate-sized effects on intestinal barrier function, with 2 reaching statistical significance. However, most studies were preclinical (cell or mouse models), and the interventions varied (including different probiotic strains and postbiotics), so direct evidence for Lactobacillus rhamnosus specifically is limited.

Caveats: Evidence base is small (only 4 studies) — conclusions should be considered preliminary. Available evidence is overwhelmingly positive — clinical literature in this area is subject to publication bias (null-result studies are less likely to be published or indexed). Additionally, most studies are preclinical and/or used strains or substances other than Lactobacillus rhamnosus, limiting direct applicability.

Generated Jun 11, 2026

4 of 4 papers

Effects of Lactobacillus paracei JY062 Postbiotic on Intestinal Barrier, Immunity, and Gut Microbiota.
- 2025-04-05
- Nutrients 17(7)
- - Jinfeng Guo
  - Ying Zhao
  - Wenqian Guo
  - Yilin Sun
  - Wei Zhang
  - Qianyu Zhao
  - Yu Zhang
  - Yujun Jiang
- PubMed: 40219029
- DOI: 10.3390/nu17071272
4 Insights on
- Improved Intestinal Barrier Function
- Low evidence
- Rigorous Journal
It prevented LPS-induced TEER reduction in Caco-2 monolayers, decreased FITC-dextran permeability, restored intestinal microvilli integrity, and upregulated tight junction genes (ZO-1, occludin, claudin-1, and E-cadherin).
Effect
Beneficial
Effect size
Moderate
Lactiplantibacillus plantarum NMGL2 exopolysaccharide ameliorates DSS-induced IBD in mice mainly by regulation of intestinal tight junction and NF-κB p65 protein expression.
- 2024-10-28
- Frontiers in microbiology 15
- - Zengjia Zhou
  - Min Zhang
  - Mengke Yao
  - Jasra Naseeb
  - Abid Sarwar
  - Zhennai Yang
  - Tariq Aziz
  - Majid Alhomrani
  - Walaa F Alsanie
  - Abdulhakeem S Alamri
- PubMed: 39529667
- DOI: 10.3389/fmicb.2024.1491727
4 Insights on
- Improved Intestinal Barrier Function
- Low evidence
significantly (p < 0.05) enhanced expression of ZO-1 and occludin
Effect
Beneficial
Effect size
Moderate
Significant
Yes
Three important short-chain fatty acids (SCFAs) attenuate the inflammatory response induced by 5-FU and maintain the integrity of intestinal mucosal tight junction.
- 2022-04-21
- BMC immunology 23(1)
- - Xi Yue
  - Sun Wen
  - Ding Long-Kun
  - Yan Man
  - Sun Chang
  - Zhang Min
  - Li Shuang-Yu
  - Qian Xin
  - Ma Jie
  - Wu Liang
- PubMed: 35448938
- DOI: 10.1186/s12865-022-00495-3
2 Insights on
- Improved Intestinal Barrier Function
- Low evidence
The expressions of ZO-1 and Occludin in intestinal mucosa were significantly increased.
Effect
Beneficial
Effect size
Moderate
Significant
Yes
A Multi-Strain Probiotic Formulation Improves Intestinal Barrier Function by the Modulation of Tight and Adherent Junction Proteins.
- 2022-08-22
- Cells 11(16)
- - Raffaella di Vito
  - Carmela Conte
  - Giovanna Traina
- PubMed: 36010692
- DOI: 10.3390/cells11162617
2 Insights on
- Improved Intestinal Barrier Function
- Low evidence
The probiotic formulation tested in this study modulates the expression of TJPs and prevents inflammatory damage. Our findings provide new insights into the mechanisms by which probiotics are able to prevent damage to the gut epithelial barrier.
Effect
Beneficial
Effect size
Moderate

Lactobacillus rhamnosus and Improved Intestinal Barrier Function

Effects of Lactobacillus paracei JY062 Postbiotic on Intestinal Barrier, Immunity, and Gut Microbiota.

Lactiplantibacillus plantarum NMGL2 exopolysaccharide ameliorates DSS-induced IBD in mice mainly by regulation of intestinal tight junction and NF-κB p65 protein expression.

Three important short-chain fatty acids (SCFAs) attenuate the inflammatory response induced by 5-FU and maintain the integrity of intestinal mucosal tight junction.

A Multi-Strain Probiotic Formulation Improves Intestinal Barrier Function by the Modulation of Tight and Adherent Junction Proteins.