A Pilot Study of the Effect of Lactobacillus casei Obtained from Long-Lived Elderly on Blood Biochemical, Oxidative, and Inflammatory Markers, and on Gut Microbiota in Young Volunteers
- 2021-10-29
- Nutrients 13(11)
- Lijun Mei
- Wenlong Zheng
- Zheng-Tao Zhao
- N. Meng
- Qin-Ren Zhang
- Wenyang Zhu
- Rui-Ding Li
- Xiao-Lin Liang
- Quanyang Li
- PubMed: 34836153
- DOI: 10.3390/nu13113891
Abstract
Probiotic intake has been shown to improve certain physiological health indicators. We aimed to examine effects of Lactobacillus casei LTL1879, obtained from long-lived elderly volunteers, on blood biochemical, oxidative, and inflammatory markers and gut microbiota in twenty healthy, young volunteers. Volunteers were randomly divided into equal probiotic and placebo groups and changes in blood biochemical indicators, oxidative and inflammatory markers, and gut microbiota were examined after three weeks of probiotic intervention. The probiotic group's antioxidant levels were significantly enhanced post-intervention. Total antioxidant capacity (T-AOC) levels were significantly increased (p < 0.0001), while malondialdehyde (MDA) levels decreased (p < 0.05), and total superoxide dismutase (T-SOD) levels increased, but with no significant difference. In addition, Interleukin-10 (IL-10) and tumor necrosis factor-α (TNF-α) levels were significantly up-regulated and down-regulated (p < 0.05, respectively). Escherichia coli, Enterococcus, and Bacteroides expression was significantly reduced (p < 0.05), while Clostridium leptum, Bifidobacterium, and Lactobacillus expression increased (p < 0.05). Volunteer health status was quantified using principal components and cluster analysis, indicating that the probiotic group's overall score was higher than that of the placebo group. The results of this pilot study suggest L. casei LTL 1879 can significantly improve specific immune, oxidative, and gut microbiota characteristics related to health factors.
Keywords: Lactobacillus casei LTL1879; blood biochemical; gut microbiota; healthy young people; inflammation; oxidant; probiotics.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus casei | Altered Gut Microbiota Composition | Beneficial | Large |
| Lactobacillus casei | Improved Antioxidant Levels | Beneficial | Large |
| Lactobacillus casei | Improved Overall Health Status | Beneficial | Moderate |
| Lactobacillus casei | Regulated Inflammation | Beneficial | Moderate |
| Lactobacillus casei HA-108 | Elevated Antioxidant Levels | Beneficial | Large |
| Lactobacillus casei HA-108 | Increased Interleukin-10 Levels | Beneficial | Small |
| Lactobacillus casei HA-108 | Increased Superoxide Dismutase Activity | Beneficial | Small |
| Lactobacillus casei HA-108 | Reduced Serum Malondialdehyde Levels | Beneficial | Small |
| Lactobacillus casei HA-108 | Reduced TNF-α Levels | Beneficial | Small |
| Lactobacillus casei R0215 | Elevated Interleukin-10 Levels | Beneficial | Small |
| Lactobacillus casei R0215 | Improved Health Status Score | Beneficial | Moderate |
| Lactobacillus casei R0215 | Improved Total Antioxidant Capacity | Beneficial | Large |
| Lactobacillus casei R0215 | Increased Bifidobacterium Levels | Beneficial | Small |
| Lactobacillus casei R0215 | Increased Clostridium leptum Expression | Beneficial | Small |
| Lactobacillus casei R0215 | Increased Superoxide Dismutase Activity | Beneficial | Small |
| Lactobacillus casei R0215 | Reduced Bacteroides Expression | Beneficial | Small |
| Lactobacillus casei R0215 | Reduced Enterococcus Expression | Beneficial | Small |
| Lactobacillus casei R0215 | Reduced Escherichia coli Expression | Beneficial | Small |
| Lactobacillus casei R0215 | Reduced Serum Malondialdehyde Levels | Beneficial | Small |
| Lactobacillus casei R0215 | Reduced Tumor Necrosis Factor Alpha | Beneficial | Small |
| Lactobacillus casei subsp. casei | Elevated Interleukin-10 Levels | Beneficial | Small |
| Lactobacillus casei subsp. casei | Improved Antioxidant Capacity | Beneficial | Large |
| Lactobacillus casei subsp. casei | Improved Overall Health Status | Beneficial | Moderate |
| Lactobacillus casei subsp. casei | Increased Superoxide Dismutase Activity | Beneficial | Small |
| Lactobacillus casei subsp. casei | Reduced Serum Malondialdehyde Levels | Beneficial | Small |
| Lactobacillus casei subsp. casei | Reduced TNF-α Levels | Beneficial | Small |
| Lactobacillus casei UALc-03 | Elevated Antioxidant Levels | Beneficial | Large |
| Lactobacillus casei UALc-03 | Improved Gut Microbiome Composition | Beneficial | Moderate |
| Lactobacillus casei UALc-03 | Improved Overall Health Status | Beneficial | Moderate |
| Lactobacillus casei UALc-03 | Increased Anti-Inflammatory Marker Levels | Beneficial | Moderate |
| Lactobacillus casei VK4 | Elevated Antioxidant Levels | Beneficial | Large |
| Lactobacillus casei VK4 | Improved Inflammatory Response | Beneficial | Moderate |
| Lactobacillus casei VK4 | Improved Overall Health Status | Beneficial | Moderate |
| Lactobacillus casei VK4 | Reduced Hepatic Oxidative Stress | Beneficial | Moderate |
| Lactobacillus sakei proBio65 | Elevated Antioxidant Levels | Beneficial | Large |
| Lactobacillus sakei proBio65 | Improved Gut Microbiota Profile | Beneficial | Moderate |
| Lactobacillus sakei proBio65 | Improved Inflammatory Biomarkers | Beneficial | Moderate |
| Lactobacillus sakei proBio65 | Improved Overall Health Status | Beneficial | Moderate |