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"Bifidobacterium longum-reactive T helper cells as marker for intestinal barrier impairment in ICU patients with sepsis".

  • 2025-12-24
  • Gut pathogens 18(1)
    • Lea-Maxie Haag
    • Markus Müller
    • Jörn Ziegler
    • Malte Lehmann
    • Julia Hecker
    • Rainer Glauben
    • Markus M Heimesaat
    • Friederike Compton
    • Britta Siegmund

Study Design

Type
Observational
Sample size
n = 19
Population
70 ICU patients, including those with sepsis, those not meeting sepsis criteria, and COVID-19 patients, as well as 20 healthy volunteers
Methods
Antigen-reactive T-cell enrichment (ARTE) technique followed by flow cytometric analysis

Background

Critical illness often leads to the development of intestinal dysbiosis, which can have a significant impact on disease outcome. Intestinal barrier dysfunction is a common problem in intensive care unit patients, particularly those with sepsis. Despite its importance, early and reliable diagnosis of barrier dysfunction and evaluation of therapeutic options remain lacking in clinical practice. Given that intestinal hyperpermeability is associated with increased translocation of luminal antigens and subsequent priming of naïve T cells, we hypothesized that analysis of circulating peripheral antigen-reactive T cells could provide insight into the functionality of the intestinal barrier.

Results

To test this hypothesis, 70 ICU patients were enrolled, including those with sepsis, those not meeting sepsis criteria, and COVID-19 patients, as well as 20 healthy volunteers. We identified a sepsis-specific T-helper cell signature in peripheral blood using the antigen-reactive T-cell enrichment (ARTE) technique followed by flow cytometric analysis. This signature was characterized by an expansion of gut trophic Bifidobacterium longum-reactive T-helper cells, indicating significant intestinal barrier dysfunction during sepsis.

Conclusion

This approach allows the study of intestinal barrier functionality and provides a means to monitor the effects of potential therapeutic interventions over time using blood samples.

Research Insights

  • Bifidobacterium longum→Impaired Intestinal Barrier Function

    we identified a sepsis-specific T-helper cell signature in peripheral blood ... characterized by an expansion of gut trophic Bifidobacterium longum-reactive T-helper cells, indicating significant intestinal barrier dysfunction during sepsis.

    Effect
    Neutral
    Effect size
    Small
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