Characterisation of Lactobacillus fermentum SM-7 isolated from koumiss, a potential probiotic bacterium with cholesterol-lowering effects.

2010-10-28
Journal of the Science of Food and Agriculture 91(3)
- D. Pan
- Xiaoqun Zeng
- Yuhua Yan

PubMed: 21218486
DOI: 10.1002/jsfa.4214

Study Design

Type: Clinical Trial
Population: ICR mice with artificially induced hyperlipidaemia
Methods: In vivo experiment with hyperlipidaemial mice.

Highly Cited
Animal Study

Abstract

Background: Lactic acid bacteria (LAB) have been demonstrated to have cholesterol-reducing effects in many studies.

Results: Lactobacillus fermentum SM-7 screened from ten LAB strains isolated from koumiss, a fermented milk drink, reduced cholesterol by 66.8%. It also showed acid and bile tolerance as well as antimicrobial activity against pathogenic Escherchia coli and Staphylococcus aureus. Lactobacillus fermentum SM-7 cells assimilated 61.5% and co-precipitated and absorbed 38.5% of the cholesterol in the media. Co-precipitation of cholesterol with cholic acid increased rapidly at pH levels below 6. In vivo experiments using L. fermentum SM-7 on artificially induced hyperlipidaemial ICR mice significantly decreased serum total cholesterol and total triglyceride levels, low-density lipoprotein cholesterol concentrations and atherogenic index (P < 0.01), while serum high-density lipoprotein cholesterol concentrations did not increase significantly (P > 0.05). The body weight and liver weight/body weight ratio of SM-7 groups were lower than those of mice on a high-cholesterol diet that were not given lactobacilli. There was no bacterial translocation in the liver, spleen or kidney of experimental mice.

Conclusion: The results suggested that L. fermentum SM-7 is a potential probiotic bacterium with cholesterol-lowering effects.

Research Insights

Lactobacillus fermentum LF61→Reduced Atherogenic Index
In vivo experiments using L. fermentum SM-7 on artificially induced hyperlipidaemial ICR mice significantly decreased ... atherogenic index.
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum LF61→Reduced Body Weight
The body weight and liver weight/body weight ratio of SM-7 groups were lower than those of mice on a high-cholesterol diet that were not given lactobacilli.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum LF61→Reduced Liver Weight-to-Body Weight Ratio
The liver weight/body weight ratio of SM-7 groups were lower than those of mice on a high-cholesterol diet that were not given lactobacilli.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum LF61→Reduced Low-Density Lipoprotein Cholesterol
In vivo experiments using L. fermentum SM-7 on artificially induced hyperlipidaemial ICR mice significantly decreased low-density lipoprotein cholesterol concentrations (P < 0.01).
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum LF61→Reduced Serum Total Cholesterol
In vivo experiments using L. fermentum SM-7 on artificially induced hyperlipidaemial ICR mice significantly decreased serum total cholesterol levels (P < 0.01).
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum LF61→Reduced Serum Triglyceride Levels
In vivo experiments using L. fermentum SM-7 on artificially induced hyperlipidaemial ICR mice significantly decreased total triglyceride levels (P < 0.01).
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum LF61→Reduced Total Cholesterol Levels
Lactobacillus fermentum SM-7 screened from ten LAB strains isolated from koumiss, a fermented milk drink, reduced cholesterol by 66.8%.
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum LF61→Stable High-Density Lipoprotein Cholesterol Level
Serum high-density lipoprotein cholesterol concentrations did not increase significantly (P > 0.05).
Effect
Neutral
Effect size
Small
Lactobacillus fermentum MAK20L13F→Improved Lipid Profile
In vivo experiments using L. fermentum SM-7 on artificially induced hyperlipidaemial ICR mice significantly decreased serum total cholesterol and total triglyceride levels, low-density lipoprotein cholesterol concentrations and atherogenic index (P < 0.01)...
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum MAK20L13F→Maintained High-Density Lipoprotein Levels
...while serum high-density lipoprotein cholesterol concentrations did not increase significantly (P > 0.05).
Effect
Neutral
Effect size
Small
Lactobacillus fermentum MAK20L13F→Reduced Bacterial Translocation
There was no bacterial translocation in the liver, spleen or kidney of experimental mice.
Effect
Neutral
Effect size
Large
Lactobacillus fermentum MAK20L13F→Reduced Body Weight
The body weight and liver weight/body weight ratio of SM-7 groups were lower than those of mice on a high-cholesterol diet that were not given lactobacilli.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum MAK20L13F→Reduced Pathogen Activity
It also showed acid and bile tolerance as well as antimicrobial activity against pathogenic Escherchia coli and Staphylococcus aureus.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum MAK20L13F→Reduced Total Cholesterol Levels
Lactobacillus fermentum SM-7 screened from ten LAB strains isolated from koumiss, a fermented milk drink, reduced cholesterol by 66.8%.
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum UALf-32→Improved Lipid Profile
In vivo experiments using L. fermentum SM-7 on artificially induced hyperlipidaemial ICR mice significantly decreased serum total cholesterol and total triglyceride levels, low-density lipoprotein cholesterol concentrations and atherogenic index (P < 0.01)...
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum UALf-32→Reduced Bacterial Translocation
There was no bacterial translocation in the liver, spleen or kidney of experimental mice.
Effect
Neutral
Effect size
Large
Lactobacillus fermentum UALf-32→Reduced Body Weight
The body weight and liver weight/body weight ratio of SM-7 groups were lower than those of mice on a high-cholesterol diet that were not given lactobacilli.
Effect
Beneficial
Effect size
Moderate
Lactobacillus fermentum UALf-32→Reduced Total Cholesterol Levels
Lactobacillus fermentum SM-7 screened from ten LAB strains isolated from koumiss, a fermented milk drink, reduced cholesterol by 66.8%.
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum VPro 14→Reduced Atherogenic Index
In vivo experiments using L. fermentum SM-7 on artificially induced hyperlipidaemial ICR mice significantly decreased ... atherogenic index.
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum VPro 14→Reduced LDL Cholesterol
In vivo experiments using L. fermentum SM-7 on artificially induced hyperlipidaemial ICR mice significantly decreased serum total cholesterol and total triglyceride levels, low-density lipoprotein cholesterol concentrations and atherogenic index (P < 0.01), while serum high-density lipoprotein cholesterol concentrations did not increase significantly (P > 0.05).
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum VPro 14→Reduced Serum Triglyceride Levels
In vivo experiments using L. fermentum SM-7 on artificially induced hyperlipidaemial ICR mice significantly decreased total triglyceride levels (P < 0.01).
Effect
Beneficial
Effect size
Large
Lactobacillus fermentum VPro 14→Reduced Total Cholesterol Levels
Lactobacillus fermentum SM-7 screened from ten LAB strains isolated from koumiss, a fermented milk drink, reduced cholesterol by 66.8%.
Effect
Beneficial
Effect size
Large