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Chronic administration of a microencapsulated probiotic enhances the bioavailability of orange juice flavanones in humans.

  • 2015-07
  • Free radical biology & medicine 84
    • Gema Pereira-Caro
    • Christine M Oliver
    • Rangika Weerakkody
    • Tanoj Singh
    • Michael Conlon
    • Gina Borges
    • Luz Sanguansri
    • Trevor Lockett
    • Susan A Roberts
    • Alan Crozier
    • Mary Ann Augustin

Study Design

Type
Clinical Trial
Population
healthy volunteers
Methods
Acute study: volunteers drank orange juice (OJ) with and without microencapsulated Bifidobacterium longum R0175; chronic study: OJ consumed after daily probiotic supplementation over 4 weeks
Duration
4 weeks
Funding
Unclear
Orange juice (OJ) flavanones are bioactive polyphenols that are absorbed principally in the large intestine. Ingestion of probiotics has been associated with favorable changes in the colonic microflora. The present study examined the acute and chronic effects of orally administered Bifidobacterium longum R0175 on the colonic microflora and bioavailability of OJ flavanones in healthy volunteers. In an acute study volunteers drank OJ with and without the microencapsulated probiotic, whereas the chronic effects were examined when OJ was consumed after daily supplementation with the probiotic over 4 weeks. Bioavailability, assessed by 0-24h urinary excretion, was similar when OJ was consumed with and without acute probiotic intake. Hesperetin-O-glucuronides, naringenin-O-glucuronides, and hesperetin-3'-O-sulfate were the main urinary flavanone metabolites. The overall urinary excretion of these metabolites after OJ ingestion and acute probiotic intake corresponded to 22% of intake, whereas excretion of key colon-derived phenolic and aromatic acids was equivalent to 21% of the ingested OJ (poly)phenols. Acute OJ consumption after chronic probiotic intake over 4 weeks resulted in the excretion of 27% of flavanone intake, and excretion of selected phenolic acids also increased significantly to 43% of (poly)phenol intake, corresponding to an overall bioavailability of 70%. Neither the probiotic bacterial profiles of stools nor the stool moisture, weight, pH, or levels of short-chain fatty acids and phenols differed significantly between treatments. These findings highlight the positive effect of chronic, but not acute, intake of microencapsulated B. longum R0175 on the bioavailability of OJ flavanones.

Research Insights

  • Bifidobacterium longum R0175→Improved Fecal Bacterial Profile

    Neither the probiotic bacterial profiles of stools nor the stool moisture, weight, pH, or levels of short-chain fatty acids and phenols differed significantly between treatments.

    Effect
    Neutral
    Effect size
    Small
    Dose
    microencapsulated probiotic, daily supplementation over 4 weeks
  • Bifidobacterium longum R0175→Improved Phenol Level

    Neither the probiotic bacterial profiles of stools nor the stool moisture, weight, pH, or levels of short-chain fatty acids and phenols differed significantly between treatments.

    Effect
    Neutral
    Effect size
    Small
    Dose
    microencapsulated probiotic, daily supplementation over 4 weeks
  • Bifidobacterium longum R0175→Improved Stool Moisture

    Neither the probiotic bacterial profiles of stools nor the stool moisture, weight, pH, or levels of short-chain fatty acids and phenols differed significantly between treatments.

    Effect
    Neutral
    Effect size
    Small
    Dose
    microencapsulated probiotic, daily supplementation over 4 weeks
  • Bifidobacterium longum R0175→Improved Stool pH

    Neither the probiotic bacterial profiles of stools nor the stool moisture, weight, pH, or levels of short-chain fatty acids and phenols differed significantly between treatments.

    Effect
    Neutral
    Effect size
    Small
    Dose
    microencapsulated probiotic, daily supplementation over 4 weeks
  • Bifidobacterium longum R0175→Increased Flavanone Metabolite Excretion

    Acute OJ consumption after chronic probiotic intake over 4 weeks resulted in the excretion of 27% of flavanone intake, and excretion of selected phenolic acids also increased significantly to 43% of (poly)phenol intake, corresponding to an overall bioavailability of 70%.

    Effect
    Beneficial
    Effect size
    Moderate
    Dose
    microencapsulated probiotic, daily supplementation over 4 weeks
  • Bifidobacterium longum R0175→Increased Short-Chain Fatty Acid Levels

    Neither the probiotic bacterial profiles of stools nor the stool moisture, weight, pH, or levels of short-chain fatty acids and phenols differed significantly between treatments.

    Effect
    Neutral
    Effect size
    Small
    Dose
    microencapsulated probiotic, daily supplementation over 4 weeks
  • Bifidobacterium longum R0175→Increased Stool Volume

    Neither the probiotic bacterial profiles of stools nor the stool moisture, weight, pH, or levels of short-chain fatty acids and phenols differed significantly between treatments.

    Effect
    Neutral
    Effect size
    Small
    Dose
    microencapsulated probiotic, daily supplementation over 4 weeks
  • Bifidobacterium longum R0175→Reduced Flavanone Metabolite Excretion

    Bioavailability, assessed by 0-24h urinary excretion, was similar when OJ was consumed with and without acute probiotic intake.

    Effect
    Neutral
    Effect size
    Small
    Dose
    microencapsulated probiotic, daily supplementation over 4 weeks
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