Construction of a Bacteriophage-Resistant Derivative of Lactococcus lactis subsp. lactis 425A by Using the Conjugal Plasmid pNP40
- 1991-12
- Applied and Environmental Microbiology 57(12)
- A. Harrington
- C. Hill
- PubMed: 16348595
- DOI: 10.1128/aem.57.12.3405-3409.1991
Study Design
- Methods
- Controlled experimental study
Abstract
Lactococcus lactis subsp. lactis 425A is an atypical strain which excretes a high concentration of alpha-acetolactate when grown in milk. The conjugative lactococcal plasmid pNP40, which encodes phage and nisin resistance, was introduced to strain 425A by conjugation, using resistance to phage and nisin as a selection. No phage-nisin resistance mutants were encountered. Transconjugants display complete resistance at both 21 and 39 degrees C to those phage previously identified as lytic for 425A. Transconjugants lose their resistance characteristics when spontaneously cured of pNP40. The commercially important property of 425A-production of high levels of alpha-acetolactic acid-is unaffected by the presence of pNP40.