Description of the draft genome sequence of Streptococcus salivarius eK12, a derivative of the probiotic strain Streptococcus salivarius K12 with a modified plasmid.

2025-07-08
Microbiology resource announcements 14(8)
- Francesco Di Pierro
- Aswin Thacharodi
- Muthiah Kumaraswami
- Alexander Bertuccioli
- Maria Laura Tanda
- Nicola Zerbinati

PubMed: 40626761
DOI: 10.1128/mra.00355-25

Study Design

Methods: The draft genome of Streptococcus salivarius eK12, a derivative of the strain K12 with a modified plasmid (pRSSL1), is presented.

The draft genome of Streptococcus salivarius eK12, a derivative of the strain K12 with a modified plasmid (pRSSL1), is presented. eK12 was engineered to enhance its probiotic properties against S. pyogenes (GAS) by modulating salivabactin production and interfering with a GAS quorum sensing pathway.

Research Insights

Streptococcus salivarius→Reduced Streptococcus pyogenes Colonization
eK12 was engineered to enhance its probiotic properties against S. pyogenes (GAS) by modulating salivabactin production and interfering with a GAS quorum sensing pathway.
Effect
Beneficial
Effect size
Moderate
Streptococcus salivarius K12→Reduced Risk of S. pyogenes Infection
eK12 was engineered to enhance its probiotic properties against S. pyogenes (GAS) by modulating salivabactin production and interfering with a GAS quorum sensing pathway.
Effect
Beneficial
Effect size
Moderate