Diarylheptanoid derivatives with α-synuclein aggregation inhibitory activities from the rhizome of Alpinia officinarum.
- 2026-06
- Fitoterapia 191
- Xue-Yi Li
- Jun-Feng She
- Qiu-Mei Lv
- Li Min
- Shi-Qin Zhang
- Lei Shi
- Xiao-Jun Huang
- Ying Wang
- Lei Wang
- Jian-Guo Song
- Chun-Tao Che
- Wen-Cai Ye
- Zhen-Long Wu
- PubMed: 42000082
- DOI: 10.1016/j.fitote.2026.107250
Study Design
- Methods
- Isolation and identification of diarylheptanoid derivatives from Alpinia officinarum; evaluation of α-synuclein aggregation inhibitory activities using native mass spectrometry and fluorescence-based assays.
Seven undescribed diarylheptanoid derivatives, alpinins A - G (1-7), together with nine known ones (8-16), were isolated and identified from the rhizomes of Alpinia officinarum Hance. Architecturally, compounds 1-4 represent four unusual dimeric diarylheptanoids featuring distinct linkage patterns, while compounds 5 and 6 are newly identified adducts consisting of diarylheptanoid motifs with a sesquiterpene and a monoterpene unit, respectively. The structures and absolute configurations of these compounds were elucidated by comprehensive spectroscopic data, DFT-based DP4+ analysis, as well as electronic circular dichroism (ECD) calculations. Additionally, the α-synuclein aggregation inhibitory activities of these isolates were evaluated using native mass spectrometry and fluorescence-based assays. Among them, compounds 7, 10, 12, and 13 exhibited significant inhibitory effects on α-synuclein aggregation and showed potent neuroprotective activities against α-synuclein aggregate-induced cytotoxicity in SH-SY5Y cells.