Dietary Lactobacillus rhamnosus GG Supplementation Improves the Mucosal Barrier Function in the Intestine of Weaned Piglets Challenged by Porcine Rotavirus
- 2016-01-04
- PLOS ONE 11(1)
- X. Mao
- Changsong Gu
- Haiyan Hu
- Jun Tang
- Daiwen Chen
- B. Yu
- Jun He
- Jie Yu
- Junqiu Luo
- G. Tian
- PubMed: 26727003
- DOI: 10.1371/journal.pone.0146312
Abstract
Lactobacillus rhamnosus GG (LGG) has been regarded as a safe probiotic strain. The aim of this study was to investigate whether dietary LGG supplementation could alleviate diarrhea via improving jejunal mucosal barrier function in the weaned piglets challenged by RV, and further analyze the potential roles for apoptosis of jejunal mucosal cells and intestinal microbiota. A total of 24 crossbred barrows weaned at 21 d of age were assigned randomly to 1 of 2 diets: the basal diet and LGG supplementing diet. On day 11, all pigs were orally infused RV or the sterile essential medium. RV infusion increased the diarrhea rate, increased the RV-Ab, NSP4 and IL-2 concentrations and the Bax mRNA levels of jejunal mucosa (P<0.05), decreased the villus height, villus height: crypt depth, the sIgA, IL-4 and mucin 1 concentrations and the ZO-1, occludin and Bcl-2 mRNA levels of jejunal mucosa (P<0.05), and affected the microbiota of ileum and cecum (P<0.05) in the weaned pigs. Dietary LGG supplementation increased the villus height and villus height: crypt depth, the sIgA, IL-4, mucin 1 and mucin 2 concentrations, and the ZO-1, occludin and Bcl-2 mRNA levels of the jejunal mucosa (P<0.05) reduced the Bax mRNA levels of the jejunal mucosa (P<0.05) in weaned pigs. Furthermore, dietary LGG supplementation alleviated the increase of diarrhea rate in the weaned pigs challenged by RV (P<0.05), and relieve the effect of RV infection on the villus height, crypt depth and the villus height: crypt depth of the jejunal mucosa (P<0.05), the NSP4, sIgA, IL-2, IL-4, mucin 1 and mucin 2 concentrations of jejunal mucosa (P<0.05), the ZO-1, occludin, Bax and Bcl-2 mRNA levels of the jejunal mucosa (P<0.05), and the microbiota of ileum and cecum (P<0.05) in the weaned pigs challenged by RV. These results suggest that supplementing LGG in diets alleviated the diarrhea of weaned piglets challenged by RV via inhibiting the virus multiplication and improving the jejunal mucosal barrier function, which was possibly due to the decreasing apoptosis of jejunal mucosal cells and the improvement of intestinal microbiota.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lacticaseibacillus rhamnosus | Improved Immunologic Barrier Function | Beneficial | Moderate |
| Lacticaseibacillus rhamnosus | Reduced Apoptosis of Jejunal Mucosal Cells | Beneficial | Moderate |
| Lacticaseibacillus rhamnosus | Reduced Diarrhea Incidence | Beneficial | Large |
| Lacticaseibacillus rhamnosus | Regulated Intestinal Microbiota | Beneficial | Moderate |
| Lactobacillus rhamnosus | Improved Intestinal Barrier Function | Beneficial | Large |
| Lactobacillus rhamnosus | Improved Intestinal Microbiota | Beneficial | Moderate |
| Lactobacillus rhamnosus | Reduced Apoptosis of Jejunal Mucosal Cells | Beneficial | Moderate |
| Lactobacillus rhamnosus | Reduced Diarrhea Rate | Beneficial | Large |
| Lactobacillus rhamnosus CLR1505 | Improved Intestinal Barrier Function | Beneficial | Large |
| Lactobacillus rhamnosus CLR1505 | Improved Intestinal Microbial Composition | Beneficial | Large |
| Lactobacillus rhamnosus CLR1505 | Reduced Apoptosis of Jejunal Mucosal Cells | Beneficial | Moderate |
| Lactobacillus rhamnosus CLR1505 | Reduced Diarrhea Incidence | Beneficial | Large |
| Lactobacillus rhamnosus CRL 1505 | Improved Intestinal Barrier Function | Beneficial | Large |
| Lactobacillus rhamnosus CRL 1505 | Improved Intestinal Microbial Composition | Beneficial | Large |
| Lactobacillus rhamnosus CRL 1505 | Reduced Apoptosis of Jejunal Mucosal Cells | Beneficial | Moderate |
| Lactobacillus rhamnosus CRL 1505 | Reduced Diarrhea Incidence | Beneficial | Large |
| Lactobacillus rhamnosus DSM33560 | Improved Intestinal Barrier Function | Beneficial | Large |
| Lactobacillus rhamnosus DSM33560 | Improved Intestinal Microbiota | Beneficial | Moderate |
| Lactobacillus rhamnosus DSM33560 | Reduced Apoptosis of Jejunal Mucosal Cells | Beneficial | Moderate |
| Lactobacillus rhamnosus DSM33560 | Reduced Diarrhea Incidence | Beneficial | Large |
| Lactobacillus rhamnosus GG | Improved Immunologic Barrier Function | Beneficial | Moderate |
| Lactobacillus rhamnosus GG | Improved Intestinal Microbiota | Beneficial | Moderate |
| Lactobacillus rhamnosus GG | Reduced Apoptosis of Jejunal Mucosal Cells | Beneficial | Moderate |
| Lactobacillus rhamnosus GG | Reduced Diarrhea Incidence | Beneficial | Large |
| Lactobacillus rhamnosus HA-11 | Reduced Inflammatory Response | Beneficial | Moderate |
| Lactobacillus rhamnosus HA-111 | Improved Intestinal Barrier Function | Beneficial | Large |
| Lactobacillus rhamnosus HA-111 | Improved Intestinal Microbiota | Beneficial | Moderate |
| Lactobacillus rhamnosus HA-111 | Reduced Diarrhea Incidence | Beneficial | Large |
| Lactobacillus rhamnosus HA-500 | Altered Cytokine Levels in Jejunal Mucosa | Beneficial | Moderate |
| Lactobacillus rhamnosus HA-500 | Improved Intestinal Barrier Function | Beneficial | Large |
| Lactobacillus rhamnosus HA-500 | Improved Intestinal Microbiota | Beneficial | Moderate |
| Lactobacillus rhamnosus HA-500 | Reduced Apoptosis of Jejunal Mucosal Cells | Beneficial | Moderate |
| Lactobacillus rhamnosus HA-500 | Reduced Diarrhea | Beneficial | Large |
| Lactobacillus rhamnosus HA-500 | Reduced Viral Load | Beneficial | Moderate |
| Lactobacillus rhamnosus IMC 501 | Improved Gut Microbiota | Beneficial | Large |
| Lactobacillus rhamnosus IMC 501 | Improved Intestinal Barrier Function | Beneficial | Large |
| Lactobacillus rhamnosus IMC 501 | Reduced Apoptosis of Jejunal Mucosal Cells | Beneficial | Moderate |
| Lactobacillus rhamnosus IMC 501 | Reduced Diarrhea Rate | Beneficial | Large |
| Lactobacillus rhamnosus LR04 | Improved Intestinal Barrier Function | Beneficial | Large |
| Lactobacillus rhamnosus LR04 | Improved Intestinal Microbiota | Beneficial | Moderate |
| Lactobacillus rhamnosus LR04 | Reduced Apoptosis of Jejunal Mucosal Cells | Beneficial | Moderate |
| Lactobacillus rhamnosus LR04 | Reduced Diarrhea Symptoms | Beneficial | Large |
| Lactobacillus rhamnosus LR05 | Improved Intestinal Barrier Function | Beneficial | Large |
| Lactobacillus rhamnosus LR05 | Improved Intestinal Microbiota | Beneficial | Moderate |
| Lactobacillus rhamnosus LR05 | Reduced Apoptosis of Jejunal Mucosal Cells | Beneficial | Moderate |
| Lactobacillus rhamnosus LR05 | Reduced Diarrhea Symptoms | Beneficial | Large |
| Lactobacillus rhamnosus LR05 | Reduced RSV Replication | Beneficial | Large |
| Lactobacillus rhamnosus LR06 | Improved Gut Microbiota | Beneficial | Large |
| Lactobacillus rhamnosus LR06 | Improved Immunologic Barrier Function | Beneficial | Moderate |
| Lactobacillus rhamnosus LR06 | Reduced Apoptosis of Jejunal Mucosal Cells | Beneficial | Moderate |
| Lactobacillus rhamnosus LR06 | Reduced Diarrhea Incidence | Beneficial | Large |
| Lactobacillus rhamnosus LRa05 | Improved Immunologic Barrier Function | Beneficial | Moderate |
| Lactobacillus rhamnosus LRa05 | Improved Intestinal Microbiota | Beneficial | Moderate |
| Lactobacillus rhamnosus LRa05 | Reduced Diarrhea Rate | Beneficial | Large |
| Lactobacillus rhamnosus lysate powder | Improved Gut Microbiota | Beneficial | Large |
| Lactobacillus rhamnosus lysate powder | Improved Intestinal Barrier Function | Beneficial | Large |
| Lactobacillus rhamnosus lysate powder | Reduced Diarrhea Symptoms | Beneficial | Large |
| Lactobacillus rhamnosus MAK79L08R | Improved Immunologic Barrier Function | Beneficial | Moderate |
| Lactobacillus rhamnosus MAK79L08R | Improved Intestinal Microbiota | Beneficial | Moderate |
| Lactobacillus rhamnosus MAK79L08R | Reduced Apoptosis of Jejunal Mucosal Cells | Beneficial | Moderate |
| Lactobacillus rhamnosus MAK79L08R | Reduced Diarrhea Incidence | Beneficial | Large |
| Lactobacillus rhamnosus NH001 | Improved Intestinal Barrier Function | Beneficial | Large |
| Lactobacillus rhamnosus NH001 | Improved Intestinal Microbiota | Beneficial | Moderate |
| Lactobacillus rhamnosus NH001 | Reduced Apoptosis of Jejunal Mucosal Cells | Beneficial | Moderate |
| Lactobacillus rhamnosus NH001 | Reduced Diarrhea Incidence | Beneficial | Large |
| Lactobacillus rhamnosus NH001 | Reduced RSV Replication | Beneficial | Large |
| Lactobacillus rhamnosus PXN 54 | Improved Intestinal Barrier Function | Beneficial | Large |
| Lactobacillus rhamnosus PXN 54 | Improved Intestinal Microbiota | Beneficial | Moderate |
| Lactobacillus rhamnosus PXN 54 | Reduced Apoptosis of Jejunal Mucosal Cells | Beneficial | Moderate |
| Lactobacillus rhamnosus PXN 54 | Reduced Diarrhea Symptoms | Beneficial | Large |
| Lactobacillus rhamnosus R0011 | Improved Immunologic Barrier Function | Beneficial | Moderate |
| Lactobacillus rhamnosus R0011 | Improved Intestinal Microbiota | Beneficial | Moderate |
| Lactobacillus rhamnosus R0011 | Reduced Apoptosis of Jejunal Mucosal Cells | Beneficial | Moderate |
| Lactobacillus rhamnosus R0011 | Reduced Diarrhea Incidence | Beneficial | Large |
| Lactobacillus rhamnosus R0343ND | Improved Immune Response | Beneficial | Moderate |
| Lactobacillus rhamnosus R0343ND | Improved Intestinal Microbiota | Beneficial | Moderate |
| Lactobacillus rhamnosus R0343ND | Improved Intestinal Mucosal Barrier Function | Beneficial | Large |
| Lactobacillus rhamnosus R0343ND | Reduced Apoptosis of Jejunal Mucosal Cells | Beneficial | Moderate |
| Lactobacillus rhamnosus R0343ND | Reduced Diarrhea Symptoms | Beneficial | Large |
| Lactobacillus rhamnosus UALr-18 | Improved Immunologic Barrier Function | Beneficial | Moderate |
| Lactobacillus rhamnosus UALr-18 | Improved Intestinal Microbiota | Beneficial | Moderate |
| Lactobacillus rhamnosus UALr-18 | Reduced Apoptosis of Jejunal Mucosal Cells | Beneficial | Moderate |
| Lactobacillus rhamnosus UALr-18 | Reduced Diarrhea Incidence | Beneficial | Large |
| Lactobacillus rhamnosus UALr-18 | Reduced Impact of Rhinovirus Infection | Beneficial | Moderate |
| Lactobacillus rhamnosus UALrh-18 | Reduced Diarrhea Rate | Beneficial | Large |