Dietary supplementation with Pediococcus pentosaceus enhances the innate immune response in and promotes growth of Litopenaeus vannamei shrimp.

2022-06-08
Journal of Fish Diseases 45(9)
- Bao Le
- Dong-geun Kim
- N. N. Phước
- Nguyen Thi Hoang Linh
- S. Yang

PubMed: 35675520
DOI: 10.1111/jfd.13666

Study Design

Type: Randomized Controlled Trial (RCT)
Population: Litopenaeus vannamei shrimp
Methods: Controlled experimental study

Rigorous Journal
Animal Study

Abstract

To reach the sustainable development goals on health management in Litopenaeus vannamei shrimp culture, Pediococcus pentosaceus AB01 was supplemented in shrimp diet. In this study, the control diet and three experimental diets containing P. pentosaceus AB01 (108 , 109 , 1010 CFU/g) were separately introduced to L. vannamei for a 28 days feeding trial. After the feeding trial, percent weight gain, feeding efficiency, and feed conversion ratio (FCR) were significantly elevated in L. vannamei administered with P. pentosaceus AB01 at 109 and 1010 colony-forming unit (CFU)/g. Protease, amylase, and trypsin were found at higher levels in the probiotic-supplied groups. The feeding of shrimps with P. pentosaceus AB01 significantly increased innate immune response and levels of related biochemical parameters in the haemolymph. After the white spot syndrome virus (WSSV) challenge, supplementation of P. pentosaceus AB01 had significant positive effects (p < .05) on survival rate, compared to that of the control diet. The higher resistance of L. vannamei to WSSV might have been due to alterations in the gut microbiome composition and upregulation of the Toll-Like Receptor (TLR) signalling pathway. Hence, P. pentosaceus AB01 may be a promising alternative feed to promote growth rate, modulate microbiota composition, and enhance immunity in L. vannamei shrimp.

Keywords: Litopenaeus vannamei; Pediococcus pentosaceus; innate immune response; microbiota; white spot syndrome virus.

Research Insights

Pediococcus pentosaceus KABP-041→Enhanced Innate Immune Function
The feeding of shrimps with P. pentosaceus AB01 significantly increased innate immune response and levels of related biochemical parameters in the haemolymph.
Effect
Beneficial
Effect size
Large
Pediococcus pentosaceus KABP-041→Improved Survival Rate Following WSSV Challenge
After the white spot syndrome virus (WSSV) challenge, supplementation of P. pentosaceus AB01 had significant positive effects (p < .05) on survival rate, compared to that of the control diet.
Effect
Beneficial
Effect size
Large
Pediococcus pentosaceus KABP-041→Increased Body Weight
percent weight gain, feeding efficiency, and feed conversion ratio (FCR) were significantly elevated in L. vannamei administered with P. pentosaceus AB01 at 109 and 1010 colony-forming unit (CFU)/g.
Effect
Beneficial
Effect size
Large
Pediococcus pentosaceus KABP-041→Increased Protease Levels
Protease, amylase, and trypsin were found at higher levels in the probiotic-supplied groups.
Effect
Beneficial
Effect size
Large
Pediococcus pentosaceus MAK99P15P→Improved Cell Survival Rates
After the white spot syndrome virus (WSSV) challenge, supplementation of P. pentosaceus AB01 had significant positive effects (p < .05) on survival rate, compared to that of the control diet.
Effect
Beneficial
Effect size
Large
Pediococcus pentosaceus MAK99P15P→Improved Enzyme Activity
Protease, amylase, and trypsin were found at higher levels in the probiotic-supplied groups.
Effect
Beneficial
Effect size
Large
Pediococcus pentosaceus MAK99P15P→Improved Immune Response
The feeding of shrimps with P. pentosaceus AB01 significantly increased innate immune response and levels of related biochemical parameters in the haemolymph.
Effect
Beneficial
Effect size
Large
Pediococcus pentosaceus MAK99P15P→Increased Body Weight
percent weight gain, feeding efficiency, and feed conversion ratio (FCR) were significantly elevated in L. vannamei administered with P. pentosaceus AB01 at 109 and 1010 colony-forming unit (CFU)/g.
Effect
Beneficial
Effect size
Large