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Differential intestinal anti-inflammatory effects of Lactobacillus fermentum and Lactobacillus salivarius in DSS mouse colitis: impact on microRNAs expression and microbiota composition.

  • 2017-08-29
  • Molecular Nutrition & Food Research 61(11)
    • A. Rodríguez‐Nogales
    • F. Algieri
    • J. Garrido-Mesa
    • T. Vezza
    • M. P. Utrilla
    • N. Chueca
    • Federico García
    • M. Olivares
    • M. Rodríguez-Cabezas
    • J. Gálvez

Abstract

Scope: To compare the intestinal anti-inflammatory effects of two probiotics Lactobacillus fermentum and Lactobacillus salivarius in mouse colitis, focusing on their impact on selected miRNAs and microbiota composition.

Methods and results: Male C57BL/6J mice were randomly assigned to four groups (n = 10): non-colitic, DSS colitic and two colitic groups treated with probiotics (5 × 108 CFU/mouse/day). Both probiotics ameliorated macroscopic colonic damage. They improved the colonic expression of markers involved in the immune response, and the expression of miR-155 and miR-223. L. fermentum also restored miR-150 and miR-143 expression, also linked to the preservation of the intestinal barrier function. Besides, these beneficial effects were associated with the amelioration of the microbiota dysbiosis and a recovery of the SCFAs- and lactic acid-producing bacterial populations, although only L. fermentum improved Chao richness, Pielou evenness and Shannon diversity. Moreover, L. fermentum also restored the Treg cell population in MLNs and the Th1/Th2 cytokine balance.

Conclusion: Both probiotics exerted intestinal anti-inflammatory effects in DSS-mouse colitis, maybe due to their ability to restore the intestinal microbiota homeostasis and modulate the immune response. L. fermentum showed a greater beneficial effect compared to L. salivarius, which makes it more interesting for future studies.

Keywords: DSS mouse colitis; MicroRNA; Microbiota; Probiotic; Pyrosequencing.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus fermentumImproved Immune ResponseBeneficial
Moderate
Lactobacillus fermentumImproved Macroscopic Colonic DamageBeneficial
Moderate
Lactobacillus fermentumImproved Microbial DiversityBeneficial
Moderate
Lactobacillus fermentumImproved Microbiota BalanceBeneficial
Moderate
Lactobacillus fermentumRestored Intestinal Barrier FunctionBeneficial
Moderate
Lactobacillus fermentumRestored Regulatory T Cell PopulationBeneficial
Moderate
Lactobacillus fermentum CECT5716Improved Immune Marker ExpressionBeneficial
Moderate
Lactobacillus fermentum CECT5716Improved Macroscopic Colonic DamageBeneficial
Moderate
Lactobacillus fermentum CECT5716Improved Microbiota BalanceBeneficial
Moderate
Lactobacillus fermentum CECT5716Restored Th1/Th2 Cytokine BalanceBeneficial
Moderate
Lactobacillus fermentum CECT5716Restored Treg Cell Population in MLNsBeneficial
Moderate
Lactobacillus fermentum Lf-33Improved Immune ResponseBeneficial
Moderate
Lactobacillus fermentum Lf-33Improved Macroscopic Colonic DamageBeneficial
Moderate
Lactobacillus fermentum Lf-33Improved Microbiota BalanceBeneficial
Moderate
Lactobacillus fermentum Lf-33Restored Regulatory T Cell Population in Mesenteric Lymph NodesBeneficial
Moderate
Lactobacillus fermentum Lf-33Restored Th1/Th2 Cytokine BalanceBeneficial
Moderate
Lactobacillus fermentum LF61Improved Immune ResponseBeneficial
Moderate
Lactobacillus fermentum LF61Improved Macroscopic Colonic DamageBeneficial
Moderate
Lactobacillus fermentum LF61Improved Microbial DiversityBeneficial
Moderate
Lactobacillus fermentum LF61Improved Microbiota BalanceBeneficial
Moderate
Lactobacillus fermentum LF61Restored Regulatory T Cell Population in Mesenteric Lymph NodesBeneficial
Moderate
Lactobacillus fermentum LF61Restored Th1/Th2 Cytokine BalanceBeneficial
Moderate
Lactobacillus fermentum ME-3Improved Colonic Immune ResponseBeneficial
Moderate
Lactobacillus fermentum ME-3Improved Gut Microbiota CompositionBeneficial
Large
Lactobacillus fermentum ME-3Improved Microbiota BalanceBeneficial
Moderate
Lactobacillus SalivariusImproved Immune ResponseBeneficial
Moderate
Lactobacillus SalivariusImproved Macroscopic Colonic DamageBeneficial
Moderate
Lactobacillus SalivariusImproved Microbiota BalanceBeneficial
Moderate
Lactobacillus salivarius BLIS K12Improved Colonic Immune Response Markers ExpressionBeneficial
Moderate
Lactobacillus salivarius BLIS K12Improved Gut Microbiota BalanceBeneficial
Moderate
Lactobacillus salivarius BLIS K12Improved Macroscopic Colonic DamageBeneficial
Moderate
Lactobacillus salivarius HA-118Improved Immune ResponseBeneficial
Moderate
Lactobacillus salivarius HA-118Improved Macroscopic Colonic DamageBeneficial
Moderate
Lactobacillus salivarius HA-118Improved Microbiota BalanceBeneficial
Moderate
Lactobacillus salivarius SD-5208Improved Immune ResponseBeneficial
Moderate
Lactobacillus salivarius SD-5208Improved Macroscopic Colonic DamageBeneficial
Moderate
Lactobacillus salivarius SD-5208Improved Microbiota BalanceBeneficial
Moderate
Lactobacillus salivarius UALb-07Improved Colonic Immune ResponseBeneficial
Moderate
Lactobacillus salivarius UALb-07Improved Macroscopic Colonic DamageBeneficial
Moderate
Lactobacillus salivarius UALb-07Improved Microbiota BalanceBeneficial
Moderate
Lactobacillus salivarius UALs07Improved Immune ResponseBeneficial
Moderate
Lactobacillus salivarius UALs07Improved Macroscopic Colonic DamageBeneficial
Moderate
Lactobacillus salivarius UALs07Improved Microbiota BalanceBeneficial
Moderate
Lactobacillus salivarius UALs07 UASImproved Immune ResponseBeneficial
Moderate
Lactobacillus salivarius UALs07 UASImproved Microbiota BalanceBeneficial
Moderate
Lactobacillus salivarius VPro 15Improved Colonic HealthBeneficial
Moderate
Lactobacillus salivarius VPro 15Improved Immune Marker ExpressionBeneficial
Moderate

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