Differential intestinal anti-inflammatory effects of Lactobacillus fermentum and Lactobacillus salivarius in DSS mouse colitis: impact on microRNAs expression and microbiota composition.
- 2017-08-29
- Molecular Nutrition & Food Research 61(11)
- A. Rodríguez‐Nogales
- F. Algieri
- J. Garrido-Mesa
- T. Vezza
- M. P. Utrilla
- N. Chueca
- Federico García
- M. Olivares
- M. Rodríguez-Cabezas
- J. Gálvez
- PubMed: 28752563
- DOI: 10.1002/mnfr.201700144
Study Design
- Type
- Randomized Controlled Trial (RCT)
- Sample size
- n = 10
- Population
- Male C57BL/6J mice
- Methods
- Controlled experimental study
- Highly Cited
- Animal Study
Abstract
Scope: To compare the intestinal anti-inflammatory effects of two probiotics Lactobacillus fermentum and Lactobacillus salivarius in mouse colitis, focusing on their impact on selected miRNAs and microbiota composition.
Methods and results: Male C57BL/6J mice were randomly assigned to four groups (n = 10): non-colitic, DSS colitic and two colitic groups treated with probiotics (5 × 108 CFU/mouse/day). Both probiotics ameliorated macroscopic colonic damage. They improved the colonic expression of markers involved in the immune response, and the expression of miR-155 and miR-223. L. fermentum also restored miR-150 and miR-143 expression, also linked to the preservation of the intestinal barrier function. Besides, these beneficial effects were associated with the amelioration of the microbiota dysbiosis and a recovery of the SCFAs- and lactic acid-producing bacterial populations, although only L. fermentum improved Chao richness, Pielou evenness and Shannon diversity. Moreover, L. fermentum also restored the Treg cell population in MLNs and the Th1/Th2 cytokine balance.
Conclusion: Both probiotics exerted intestinal anti-inflammatory effects in DSS-mouse colitis, maybe due to their ability to restore the intestinal microbiota homeostasis and modulate the immune response. L. fermentum showed a greater beneficial effect compared to L. salivarius, which makes it more interesting for future studies.
Keywords: DSS mouse colitis; MicroRNA; Microbiota; Probiotic; Pyrosequencing.
Research Insights
Both probiotics ameliorated macroscopic colonic damage. They improved the colonic expression of markers involved in the immune response, and the expression of miR-155 and miR-223.
- Effect
- Beneficial
- Effect size
- Moderate
Both probiotics ameliorated macroscopic colonic damage.
- Effect
- Beneficial
- Effect size
- Moderate
...although only L. fermentum improved Chao richness, Pielou evenness and Shannon diversity.
- Effect
- Beneficial
- Effect size
- Moderate
L. fermentum also restored the Treg cell population in MLNs and the Th1/Th2 cytokine balance.
- Effect
- Beneficial
- Effect size
- Moderate
L. fermentum also restored the Treg cell population in MLNs and the Th1/Th2 cytokine balance.
- Effect
- Beneficial
- Effect size
- Moderate
Both probiotics ameliorated macroscopic colonic damage. They improved the colonic expression of markers involved in the immune response, and the expression of miR-155 and miR-223.
- Effect
- Beneficial
- Effect size
- Moderate
Both probiotics ameliorated macroscopic colonic damage.
- Effect
- Beneficial
- Effect size
- Moderate
They improved the colonic expression of markers involved in the immune response, and the expression of miR-155 and miR-223.
- Effect
- Beneficial
- Effect size
- Moderate
Both probiotics ameliorated macroscopic colonic damage.
- Effect
- Beneficial
- Effect size
- Moderate
Both probiotics ameliorated macroscopic colonic damage. They improved the colonic expression of markers involved in the immune response, and the expression of miR-155 and miR-223.
- Effect
- Beneficial
- Effect size
- Moderate
Both probiotics ameliorated macroscopic colonic damage.
- Effect
- Beneficial
- Effect size
- Moderate