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Effect of Heat-Killed Lactiplantibacillus plantarum SNK12 on Sleep Quality and Stress-Related Neuroendocrine and Inflammatory Biomarkers in Adults: A Randomized, Double-Blind, Placebo-Controlled, Parallel-Group Trial.

  • 2025-12-24
  • Life (Basel, Switzerland) 16(1)
    • Takumi Watanabe
    • Shiho Kurosaka
    • Yuriko Namatame
    • Toshio Kawahara

Study Design

Type
Randomized Controlled Trial (RCT)
Population
Healthy adults
Methods
A randomized controlled trial to test whether heat-killed SNK (≥1 × 10^11 cells/day for 4 weeks) improves sleep quality and alters stress-related immune and neuroendocrine biomarkers; Healthy adults received SNK or a placebo for 4 weeks.
Heat-killed Lactiplantibacillus plantarum SNK12 (SNK), isolated from a traditional Japanese fermented food, has been suggested to influence sleep quality, but human data on sleep improvement with heat-killed lactic acid bacteria (postbiotics) remain limited. We conducted a randomized controlled trial to test whether heat-killed SNK (≥1 × 1011 cells/day for 4 weeks) improves sleep quality and alters stress-related immune and neuroendocrine biomarkers. Healthy adults received SNK or a placebo for 4 weeks. The primary outcome was the Oguri-Shirakawa-Azumi Sleep Inventory MA version (OSA-MA) factor "Sleepiness on Rising"; secondary outcomes were other OSA-MA factors and the stress-related biomarkers salivary cortisol and plasma tumor necrosis factor-α (TNF-α). Compared with placebo, SNK improved Sleepiness on Rising (p = 0.032) and Initiation and Maintenance of Sleep (p = 0.010). Salivary cortisol (p = 0.016) and plasma TNF-α (p = 0.037) were also lower with SNK, and no safety concerns emerged. These concomitant changes in subjective sleep indices and stress-related biomarkers are consistent with modulation of hypothalamic-pituitary-adrenal axis activity and inflammatory pathways along the gut-brain axis. SNK may, therefore, represent a practical postbiotic option to support sleep quality.

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