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Effect of Lactobacillus fermentum MCC2760-Based Probiotic Curd on Hypercholesterolemic C57BL6 Mice

  • 2021-03-12
  • ACS Omega 6(11)
    • Meena Kumari Palani Kumar
    • P. Halami
    • Muthukumar Serva Peddha

Abstract

Lactobacillus fermentum MCC2760 is a probiotic strain proven earlier for cholesterol-reducing and anti-inflammatory properties in vitro and in vivo. This study investigates L. fermentum MCC2760-based probiotic curd in high-cholesterol diet (HCD)-fed C57BL6 mice. The mice were grouped into normal diet control, high-cholesterol diet control, normal diet with probiotic supplementation, and high-cholesterol diet with probiotic supplementation. Control groups and treatment groups were supplemented with market curd and probiotic curd, respectively, via oral gavage for eight weeks. The probiotic count was maintained at 10.95 log CFU/mL in the developed probiotic curd. The HCD group showed an increase in feed intake and body weight. Reduction in the levels of serum cholesterol, triglycerides, low-density lipoprotein cholesterol, glucose, aspartate aminotransferase, and alanine transaminase was observed in probiotic-supplemented groups. The probiotic-supplemented group resulted in an increase in Lactobacillus spp. count along with reduced pathogen count in the feces. Probiotic supplementation also showed a reduction in the bacterial translocation count in mesenteric adipose tissue. Expression of inflammatory markers by qPCR showed the decline in the fold change of TNF-α, IL-6, and IL-12 and elevation in the fold change of IL-10 in the adipose tissue of the probiotic-treated group. Probiotic supplementation also improved the expression of GLP-1, ZO-1, and CB2 in the intestine. They were thus possibly playing a role in the enhancement of barrier function. Histopathological sections showed improvement in the cellular infiltration and pathological indications due to the high-cholesterol diet intake. Our study also confirmed that probiotics could increase serum antioxidant enzymes in treated groups, showing their beneficial antioxidant activity. It suggests the anti-inflammatory, antioxidant effect, and gut barrier function of the given probiotic formulation, which ameliorate hypercholesterolemia.

Research Insights

SupplementHealth OutcomeEffect TypeEffect Size
Lactobacillus fermentum CECT5716Improved Histopathological IndicatorsBeneficial
Moderate
Lactobacillus fermentum CECT5716Improved Intestinal Barrier FunctionBeneficial
Moderate
Lactobacillus fermentum CECT5716Increased Serum Antioxidant Enzyme LevelsBeneficial
Moderate
Lactobacillus fermentum CECT5716Reduced Bacterial Translocation in Mesenteric Adipose TissueBeneficial
Moderate
Lactobacillus fermentum CECT5716Reduced Inflammatory Marker Protein LevelsBeneficial
Large
Lactobacillus fermentum CECT5716Reduced Pathogen Count in FecesBeneficial
Moderate
Lactobacillus fermentum CECT5716Reduced Serum Total Cholesterol LevelsBeneficial
Large
Lactobacillus fermentum LF61Improved Intestinal Barrier FunctionBeneficial
Moderate
Lactobacillus fermentum LF61Increased Antioxidant ActivityBeneficial
Moderate
Lactobacillus fermentum LF61Increased Beneficial Bacteria LevelsBeneficial
Moderate
Lactobacillus fermentum LF61Reduced Bacterial TranslocationBeneficial
Moderate
Lactobacillus fermentum LF61Reduced Glucose LevelsBeneficial
Moderate
Lactobacillus fermentum LF61Reduced Inflammatory MarkersBeneficial
Large
Lactobacillus fermentum LF61Reduced Serum CholesterolBeneficial
Large
Lactobacillus fermentum MAK20L13FImproved Antioxidant Enzyme LevelsBeneficial
Large
Lactobacillus fermentum MAK20L13FImproved Blood Glucose LevelsBeneficial
Moderate
Lactobacillus fermentum MAK20L13FImproved Histopathological ProtectionBeneficial
Moderate
Lactobacillus fermentum MAK20L13FImproved Inflammatory Marker ProfileBeneficial
Large
Lactobacillus fermentum MAK20L13FImproved Intestinal Barrier FunctionBeneficial
Moderate
Lactobacillus fermentum MAK20L13FReduced Low-Density Lipoprotein LevelBeneficial
Large
Lactobacillus fermentum MAK20L13FReduced Serum Cholesterol LevelsBeneficial
Large
Lactobacillus fermentum MAK20L13FReduced Triglyceride LevelsBeneficial
Large
Lactobacillus fermentum ME-3Improved Intestinal Barrier FunctionBeneficial
Moderate
Lactobacillus fermentum ME-3Increased Anti-Inflammatory Marker (IL-10) ExpressionBeneficial
Moderate
Lactobacillus fermentum ME-3Increased Lactobacillus Species CountBeneficial
Large
Lactobacillus fermentum ME-3Increased Serum Antioxidant Enzyme LevelsBeneficial
Moderate
Lactobacillus fermentum ME-3Reduced Alanine Transaminase LevelsBeneficial
Large
Lactobacillus fermentum ME-3Reduced Aspartate Aminotransferase LevelsBeneficial
Large
Lactobacillus fermentum ME-3Reduced Glucose LevelsBeneficial
Moderate
Lactobacillus fermentum ME-3Reduced Inflammatory Marker ExpressionBeneficial
Moderate
Lactobacillus fermentum ME-3Reduced Low-Density Lipoprotein LevelBeneficial
Large
Lactobacillus fermentum ME-3Reduced Mesenteric Adipose Tissue AccumulationBeneficial
Moderate
Lactobacillus fermentum ME-3Reduced Serum CholesterolBeneficial
Large
Lactobacillus fermentum ME-3Reduced TriglyceridesBeneficial
Large

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