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Effect of Microencapsulation Techniques on the Stress Resistance and Biological Activity of Bovine Lactoferricin-Lactoferrampin-Encoding Lactobacillus reuteri.

  • 2022-10-11
  • Foods (Basel, Switzerland) 11(20)
    • Xueying Wang
    • Weichun Xie
    • Senhao Zhang
    • Yilan Shao
    • Jiyao Cai
    • Limeng Cai
    • Xiaona Wang
    • Zhifu Shan
    • Han Zhou
    • Jiaxuan Li
    • Wen Cui
    • Li Wang
    • Xinyuan Qiao
    • Yijing Li
    • Yanping Jiang
    • Lijie Tang

Study Design

Methods
selected nine kinds of wall material combinations to encapsulate LR-LFCA by spray drying microencapsulation; storage stability, microstructural morphology, biological activity, and simulated digestion in vivo or in vitro were evaluated
Bovine lactoferricin-lactoferrampin-encoding Lactobacillus reuteri (LR-LFCA) has been found to benefit its host by strengthening its intestinal barrier. However, several questions remain open concerning genetically engineered strains maintaining long-term biological activity at room temperature. In addition, probiotics are vulnerable to harsh conditions in the gut, such as acidity and alkalinity, and bile salts. Microencapsulation is a technique to entrap probiotic bacteria into gastro-resistant polymers to carry them directly to the intestine. We selected nine kinds of wall material combinations to encapsulate LR-LFCA by spray drying microencapsulation. The storage stability, microstructural morphology, biological activity, and simulated digestion in vivo or in vitro of the microencapsulated LR-LFCA were further evaluated. The results showed that LR-LFCA had the highest survival rate when microcapsules were prepared using a wall material mixture (skim milk, sodium glutamate, polyvinylpyrrolidone, maltodextrin, and gelatin). Microencapsulated LR-LFCA increased the stress resistance capacity and colonization abilities. In the present study, we have identified a suitable wall material formulation for spray-dried microencapsulation of genetically engineered probiotic products, which would facilitate their storage and transport.

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