Effect of Microencapsulation Techniques on the Stress Resistance and Biological Activity of Bovine Lactoferricin-Lactoferrampin-Encoding <i>Lactobacillus reuteri</i>.
- 2022-10-11
- Foods (Basel, Switzerland) 11(20)
- Xueying Wang
- Weichun Xie
- Senhao Zhang
- Yilan Shao
- Jiyao Cai
- Limeng Cai
- Xiaona Wang
- Zhifu Shan
- Han Zhou
- Jiaxuan Li
- Wen Cui
- Li Wang
- Xinyuan Qiao
- Yijing Li
- Yanping Jiang
- Lijie Tang
- PubMed: 37430918
- DOI: 10.3390/foods11203169
Bovine lactoferricin-lactoferrampin-encoding Lactobacillus reuteri (LR-LFCA) has been found to benefit its host by strengthening its intestinal barrier. However, several questions remain open concerning genetically engineered strains maintaining long-term biological activity at room temperature. In addition, probiotics are vulnerable to harsh conditions in the gut, such as acidity and alkalinity, and bile salts. Microencapsulation is a technique to entrap probiotic bacteria into gastro-resistant polymers to carry them directly to the intestine. We selected nine kinds of wall material combinations to encapsulate LR-LFCA by spray drying microencapsulation. The storage stability, microstructural morphology, biological activity, and simulated digestion in vivo or in vitro of the microencapsulated LR-LFCA were further evaluated. The results showed that LR-LFCA had the highest survival rate when microcapsules were prepared using a wall material mixture (skim milk, sodium glutamate, polyvinylpyrrolidone, maltodextrin, and gelatin). Microencapsulated LR-LFCA increased the stress resistance capacity and colonization abilities. In the present study, we have identified a suitable wall material formulation for spray-dried microencapsulation of genetically engineered probiotic products, which would facilitate their storage and transport.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus rhamnosus lysate powder | Improved Gut Microbiota | Beneficial | Moderate |
| Lactobacillus rhamnosus lysate powder | Improved Probiotic Product Stability | Beneficial | Moderate |
| Lactobacillus rhamnosus lysate powder | Improved Stress Resistance | Beneficial | Moderate |