Effect of oral administration involving a probiotic strain of Lactobacillus reuteri on pro-inflammatory cytokine response in patients with chronic periodontitis.

2014-02-09
Archivum immunologiae et therapiae experimentalis 62(6)
- Anna K Szkaradkiewicz
- Janina Stopa
- Tomasz M Karpiński

PubMed: 24509697
DOI: 10.1007/s00005-014-0277-y

Study Design

Type: Clinical Trial
Sample size: n = 24
Population: 38 adult patients with moderate chronic periodontitis
Methods: Professional cleaning of teeth; two weeks later, 24 patients received probiotic tablets containing L. reuteri strain (Prodentis), 14 patients received no probiotic (control group); GCF sampled and cytokines measured via ELISA
Blinding: Open-label
Funding: Unclear

This study aimed at evaluation of pro-inflammatory cytokine response (TNF-α, IL-1β and IL-17) in patients with chronic periodontitis administered per os with a probiotic strain of Lactobacillus reuteri. In the 38 adult patients with moderate chronic periodontitis, professional cleaning of teeth was performed. Two weeks after performing the oral hygienization procedures, clinical examination permitted to distinguish a group of 24 patients (Group 1) in whom treatment with probiotic tablets containing L. reuteri strain, producing hydrogen peroxide (Prodentis), was conducted. In the remaining 14 patients, no probiotic tablet treatment was applied (the control group; Group 2). From all patients in two terms, gingival crevicular fluid (GCF) was sampled from all periodontal pockets. Estimation of TNF-α, IL-lβ and IL-17 in GCF was performed using the ELISA method. After completion of the therapy with probiotic tablets, 18 (75%) of the patients of Group 1 have manifested a significant decrease in levels of studied pro-inflammatory cytokines (TNF-α, IL-1β and IL-17). In parallel, we have detected an improvement of clinical indices [sulcus bleeding index (SBI), periodontal probing depth (PPD), clinical attachment level (CAL)]. At individuals of Group 2 levels of studies, pro-inflammatory cytokines and clinical indices (SBI, PPD, CAL) were significantly higher than in Group 1. Results obtained in this study indicate that application of oral treatment with tablets containing probiotic strain of L. reuteri induces in most patients with chronic periodontitis a significant reduction of pro-inflammatory cytokine response and improvement of clinical parameters (SBI, PPD, CAL). Therefore, such an effect may result in a reduced activity of the morbid process.

Research Insights

Lactobacillus reuteri→Improved Clinical Attachment
In parallel, we have detected an improvement of clinical indices [sulcus bleeding index (SBI), periodontal probing depth (PPD), clinical attachment level (CAL)].
Effect
Beneficial
Effect size
Moderate
Dose
Probiotic tablets (Prodentis) containing L. reuteri strain producing hydrogen peroxide – exact dose not specified
Lactobacillus reuteri→Improved Periodontal Probing Depth
In parallel, we have detected an improvement of clinical indices [sulcus bleeding index (SBI), periodontal probing depth (PPD), clinical attachment level (CAL)].
Effect
Beneficial
Effect size
Moderate
Dose
Probiotic tablets (Prodentis) containing L. reuteri strain producing hydrogen peroxide – exact dose not specified
Lactobacillus reuteri→Improved Sulcus Bleeding Index
In parallel, we have detected an improvement of clinical indices [sulcus bleeding index (SBI), periodontal probing depth (PPD), clinical attachment level (CAL)].
Effect
Beneficial
Effect size
Moderate
Dose
Probiotic tablets (Prodentis) containing L. reuteri strain producing hydrogen peroxide – exact dose not specified
Lactobacillus reuteri→Reduced Interleukin-1 Beta Level
After completion of the therapy with probiotic tablets, 18 (75%) of the patients of Group 1 have manifested a significant decrease in levels of studied pro-inflammatory cytokines (TNF-α, IL-1β and IL-17).
Effect
Beneficial
Effect size
Moderate
Dose
Probiotic tablets (Prodentis) containing L. reuteri strain producing hydrogen peroxide – exact dose not specified
Lactobacillus reuteri→Reduced Interleukin-17 Level
After completion of the therapy with probiotic tablets, 18 (75%) of the patients of Group 1 have manifested a significant decrease in levels of studied pro-inflammatory cytokines (TNF-α, IL-1β and IL-17).
Effect
Beneficial
Effect size
Moderate
Dose
Probiotic tablets (Prodentis) containing L. reuteri strain producing hydrogen peroxide – exact dose not specified
Lactobacillus reuteri→Reduced Tumor Necrosis Factor Alpha
After completion of the therapy with probiotic tablets, 18 (75%) of the patients of Group 1 have manifested a significant decrease in levels of studied pro-inflammatory cytokines (TNF-α, IL-1β and IL-17).
Effect
Beneficial
Effect size
Moderate
Dose
Probiotic tablets (Prodentis) containing L. reuteri strain producing hydrogen peroxide – exact dose not specified