Effects of administration of Bifidobacterium animalis subsp. lactis GCL2505 on defecation frequency and bifidobacterial microbiota composition in humans.
- 2012-05
- Journal of Bioscience and Bioengineering 113(5)
- Akihiro Ishizuka
- K. Tomizuka
- Ryo Aoki
- Tomohiko Nishijima
- Yasuo Saito
- R. Inoue
- K. Ushida
- Takashi Mawatari
- Takayuki Ikeda
- PubMed: 22284965
- DOI: 10.1016/j.jbiosc.2011.12.016
Abstract
The aim of this study was to evaluate the changes in endogenous bifidobacteria and administered Bifidobacterium animalis subsp. lactis (B. lactis) GCL2505 (GCL2505) in the intestine after administration of GCL2505 by means of a randomized, placebo-controlled double-blind, cross-over study. An increase in the number of total bifidobacteria (the sum of B. bifidum, B. breve, B. longum subsp. longum, B. adolescentis, B. anglatum, B. catenulatum, B. pseudocatenulatum, B. dentium, B. longum subsp. infantis and B. lactis) in the feces were observed after administration of GCL2505 using species- and subspecies-specific real-time polymerase chain reaction analysis. However, the number of endogenous bifidobacteria species (excluding B. lactis) remained unchanged. B. lactis also became the predominant bifidobacterial species. Taking into account the number of GCL2505 administered, the findings further suggested that GCL2505 proliferated in the intestine. In addition, the defecation frequency increased during GCL2505 administration compared with the placebo. Moreover, a single administration study (n=17) clearly demonstrated that GCL2505 successfully reached the intestine before proliferating at least 10-fold. This is the first report to show an increase in intestinal bifidobacteria, with no changes to the endogenous species, and improvements in constipation following proliferation of administered bifidobacteria.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Bifidobacterium animalis | Improved Constipation | Beneficial | Moderate |
| Bifidobacterium animalis | Increased Bifidobacteria Levels | Beneficial | Moderate |
| Bifidobacterium animalis | Increased Defecation Frequency | Beneficial | Moderate |
| Bifidobacterium animalis | Increased Proliferation of Bifidobacterium lactis | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis | Improved Bowel Movement Frequency | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis | Increased Bifidobacteria Proliferation | Neutral | Moderate |
| Bifidobacterium animalis subsp. lactis | Increased Levels of Bifidobacteria | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis B420 | Improved Constipation | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis B420 | Increased Fecal Bifidobacteria | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis B420 | Increased Proliferation of Bifidobacterium lactis | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BLC1 | Improved Constipation | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BLC1 | Increased Bifidobacteria Proliferation | Neutral | Moderate |
| Bifidobacterium animalis subsp. lactis BLC1 | Increased Defecation Frequency | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BLC1 | Increased Intestinal Bifidobacterium Levels | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BS-01 | Improved Bowel Movement Frequency | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BS-01 | Increased Bifidobacteria Levels | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis BS-01 | Increased Bifidobacteria Proliferation | Neutral | Moderate |
| Bifidobacterium animalis subsp. lactis CECT 8145 | Improved Bowel Movement Frequency | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis CECT 8145 | Increased Intestinal Bifidobacterium Levels | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis CECT 8145 | No Change in Endogenous Bifidobacteria Levels | Neutral | Small |
| Bifidobacterium animalis subsp. lactis HN019 | Improved Bowel Movement Frequency | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis HN019 | Increased Intestinal Bifidobacteria Levels | Beneficial | Large |
| Bifidobacterium animalis subsp. lactis LAFTI B94 | Improved Bowel Movement Frequency | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis LAFTI B94 | Improved Constipation | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis LAFTI B94 | Increased Bifidobacteria Levels | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis VK2 | Improved Bowel Movement Frequency | Beneficial | Moderate |
| Bifidobacterium animalis subsp. lactis VK2 | Increased Intestinal Bifidobacteria Levels | Beneficial | Large |
| Bifidobacterium bifidum/lactis Bb-02 | Improved Bowel Movement Frequency | Beneficial | Moderate |
| Bifidobacterium bifidum/lactis Bb-02 | Increased Bifidobacteria Levels | Beneficial | Moderate |
| Bifidobacterium bifidum/lactis Bb-02 | Increased Bifidobacteria Proliferation | Neutral | Moderate |
| Bifidobacterium bifidum/lactis Bb-02 | Relieved Constipation | Beneficial | Moderate |
| Bifidobacterium lactis | Improved Bowel Movement Frequency | Beneficial | Moderate |
| Bifidobacterium lactis | Increased Intestinal Bifidobacterium Levels | Beneficial | Moderate |
| Bifidobacterium lactis B420 | Improved Bowel Movement Frequency | Beneficial | Moderate |
| Bifidobacterium lactis B420 | Increased Intestinal Bifidobacterium Levels | Beneficial | Moderate |
| Bifidobacterium lactis BI-04 | Improved Bowel Movement Frequency | Beneficial | Moderate |
| Bifidobacterium lactis BI-04 | Increased Bifidobacteria Proliferation | Neutral | Moderate |
| Bifidobacterium lactis BI-04 | Preserved Endogenous Bifidobacteria Composition | Neutral | Small |
| Bifidobacterium lactis BI-04 | Relieved Constipation | Beneficial | Moderate |
| Bifidobacterium lactis Bi-07 | Improved Bowel Movement Frequency | Beneficial | Moderate |
| Bifidobacterium lactis Bi-07 | Increased Bifidobacteria Levels | Beneficial | Moderate |
| Bifidobacterium lactis Bl-04 | Improved Bowel Movement Frequency | Beneficial | Moderate |
| Bifidobacterium lactis Bl-04 | Increased Intestinal Bifidobacterium Levels | Beneficial | Moderate |
| Bifidobacterium lactis Bl-04 | Relieved Constipation | Beneficial | Moderate |
| Bifidobacterium lactis BL818 | Improved Bowel Movement Frequency | Beneficial | Moderate |
| Bifidobacterium lactis BL818 | Increased Bifidobacterial Growth | Beneficial | Large |
| Bifidobacterium lactis BL818 | Relieved Constipation | Beneficial | Moderate |
| Bifidobacterium lactis BLA80 | Increased Bifidobacterium lactis GCL2505 Intestinal Proliferation | Beneficial | Large |
| Bifidobacterium lactis BLA80 | Increased Defecation Frequency | Beneficial | Moderate |
| Bifidobacterium lactis BLA80 | Increased Intestinal Bifidobacterium Levels | Beneficial | Moderate |
| Bifidobacterium lactis BLA80 | No Change in Endogenous Bifidobacteria Species | Neutral | Small |
| Bifidobacterium lactis BLC-1 | Increased Bifidobacteria Levels | Beneficial | Moderate |
| Bifidobacterium lactis BLC-1 | Increased Bifidobacteria Proliferation | Neutral | Moderate |
| Bifidobacterium lactis BLC-1 | Increased Defecation Frequency | Beneficial | Moderate |
| Bifidobacterium lactis BLC-1 | No Change in Endogenous Bifidobacteria Species | Neutral | Small |
| Bifidobacterium lactis BS01 | Improved Bowel Movement Frequency | Beneficial | Moderate |
| Bifidobacterium lactis BS01 | Increased Bifidobacteria Proliferation | Neutral | Moderate |
| Bifidobacterium lactis BS01 | Increased Intestinal Bifidobacterium Levels | Beneficial | Moderate |
| Bifidobacterium lactis HA-194 | Improved Bowel Movement Frequency | Beneficial | Moderate |
| Bifidobacterium lactis HA-194 | Increased Bifidobacteria Levels in Intestine | Beneficial | Large |
| Bifidobacterium lactis ISS Bif | Improved Bowel Movement Frequency | Beneficial | Moderate |
| Bifidobacterium lactis ISS Bif | Increased Bifidobacterium lactis Proliferation in the Intestine | Beneficial | Large |
| Bifidobacterium lactis ISS Bif | Increased Intestinal Bifidobacteria Population | Beneficial | Large |
| Bifidobacterium lactis ISS Bif | No Change in Endogenous Bifidobacteria Species | Neutral | Small |
| Bifidobacterium lactis MAK16B42L | Improved Bowel Movement Frequency | Beneficial | Moderate |
| Bifidobacterium lactis MAK16B42L | Increased Bacterial Proliferation | Beneficial | Large |
| Bifidobacterium lactis MAK16B42L | Increased Intestinal Bifidobacterium Levels | Beneficial | Moderate |
| Bifidobacterium lactis MAK16B42L | Increased Probiotic B. lactis Levels | Neutral | Moderate |
| Bifidobacterium lactis R101-8 | Improved Bowel Movement Frequency | Beneficial | Moderate |
| Bifidobacterium lactis R101-8 | Increased Bifidobacteria Levels | Beneficial | Moderate |
| Bifidobacterium lactis R101-8 | Increased Proliferation of Administered Bifidobacteria | Beneficial | Large |
| Bifidobacterium lactis SD-5219 | Improved Bowel Movement Frequency | Beneficial | Moderate |
| Bifidobacterium lactis SD-5219 | Increased Fecal Bifidobacteria | Beneficial | Moderate |
| Bifidobacterium lactis VK2 | Increased Bifidobacteria Levels | Beneficial | Moderate |
| Bifidobacterium lactis VK2 | No Change in Endogenous Bifidobacteria Levels | Neutral | Small |
| Bifidobacterium lactis/longum | Improved Bowel Movement Frequency | Beneficial | Moderate |
| Bifidobacterium lactis/longum | Increased Bifidobacteria Levels | Beneficial | Moderate |
| Bifidobacterium lactis/longum | Successful Gastrointestinal Tract Colonization | Beneficial | Large |