Effects of Lactobacillus plantarum and Lactobacillus paracasei on the Peripheral Immune Response in Children with Celiac Disease Autoimmunity: A Randomized, Double-Blind, Placebo-Controlled Clinical Trial
- 2019-08-16
- Nutrients 11(8)
- Å. Håkansson
- Carin Andrén Aronsson
- C. Brundin
- E. Oscarsson
- G. Molin
- D. Agardh
- PubMed: 31426299
- DOI: 10.3390/nu11081925
Abstract
Two Lactobacillus strains have proven anti-inflammatory properties by reducing pro-inflammatory responses to antigens. This randomized double-blind placebo-controlled trial tested the hypothesis that L. plantarum HEAL9 and L. paracasei 8700:2 suppress ongoing celiac disease autoimmunity in genetically at risk children on a gluten-containing diet in a longitudinally screening study for celiac disease. Seventy-eight children with celiac disease autoimmunity participated of whom 40 received 1010 CFU/day of L. plantarum HEAL9 and L. paracasei 8700:2 (probiotic group) and 38 children maltodextrin (placebo group) for six months. Blood samples were drawn at zero, three and six months and phenotyping of peripheral blood lymphocytes and IgA and IgG autoantibodies against tissue transglutaminase (tTG) were measured. In the placebo group, naïve CD45RA+ Th cells decreased (p = 0.002) whereas effector and memory CD45RO+ Th cells increased (p = 0.003). In contrast, populations of cells expressing CD4+CD25highCD45RO+CCR4+ increased in the placebo group (p = 0.001). Changes between the groups were observed for NK cells (p = 0.038) and NKT cells (p = 0.008). Median levels of IgA-tTG decreased more significantly over time in the probiotic (p = 0.013) than in the placebo (p = 0.043) group whereas the opposite was true for IgG-tTG (p = 0.062 respective p = 0.008). In conclusion, daily oral administration of L. plantarum HEAL9 and L. paracasei 8700:2 modulate the peripheral immune response in children with celiac disease autoimmunity.
Keywords: autoimmunity; celiac disease; immune function; probiotic.
Research Insights
| Supplement | Health Outcome | Effect Type | Effect Size |
|---|---|---|---|
| Lactobacillus paracasei DSM 13434 | Increased Regulatory T Cell Populations | Harmful | Moderate |
| Lactobacillus paracasei DSM 13434 | Modulated Immune Response | Beneficial | Moderate |
| Lactobacillus paracasei DSM 13434 | Reduced Median Levels of IgA-tTG | Beneficial | Moderate |
| Lactobacillus plantarum | Increased Regulatory T Cell Populations | Harmful | Moderate |
| Lactobacillus plantarum | Modulated Peripheral Immune Response | Beneficial | Moderate |
| Lactobacillus plantarum | Reduced Median Levels of IgA-tTG | Beneficial | Moderate |
| Lactobacillus plantarum DSM 6595 | Modulated Peripheral Immune Response | Beneficial | Moderate |
| Lactobacillus plantarum DSM 6595 | Reduced Median Levels of IgA-tTG | Beneficial | Moderate |
| Lactobacillus plantarum DSM 6596 | Decreased Naïve CD45RA+ T Helper Cells | Neutral | Small |
| Lactobacillus plantarum DSM 6596 | Elevated IgG-tTG Levels | Harmful | Small |
| Lactobacillus plantarum DSM 6596 | Increased Natural Killer Cell Proportion | Beneficial | Small |
| Lactobacillus plantarum DSM 6596 | Increased Natural Killer T Cells | Beneficial | Moderate |
| Lactobacillus plantarum DSM 6596 | Modulated Peripheral Immune Response | Beneficial | Moderate |
| Lactobacillus plantarum DSM 6596 | Reduced Median Levels of IgA-tTG | Beneficial | Moderate |
| Lactobacillus plantarum HEAL9 | Modulated Peripheral Immune Response | Beneficial | Moderate |
| Lactobacillus plantarum HEAL9 | Reduced Median Levels of IgA-tTG | Beneficial | Moderate |
| Lactobacillus plantarum LP09 | Change in Immunoglobulin G Tissue Transglutaminase Levels | Neutral | Small |
| Lactobacillus plantarum LP09 | Increased NK Cell Count | Neutral | Small |
| Lactobacillus plantarum LP09 | Modulated Peripheral Immune Response | Beneficial | Moderate |
| Lactobacillus plantarum LP09 | Reduced Median Levels of IgA-tTG | Beneficial | Moderate |
| Lactobacillus plantarum LP09 | Reduced Naïve CD45RA+ Th Cell Count | Beneficial | Moderate |
| Lactobacillus plantarum SD-5209 | Increased Regulatory T Cell Populations | Harmful | Moderate |
| Lactobacillus plantarum SD-5209 | Modulated Peripheral Immune Response | Beneficial | Moderate |
| Lactobacillus plantarum SD-5209 | No Change in IgG-tTG Levels | Neutral | Small |
| Lactobacillus plantarum SD-5209 | Reduced Median Levels of IgA-tTG | Beneficial | Moderate |
| Lactobacillus plantarum UALp-05M | Modulated Peripheral Immune Response | Beneficial | Moderate |
| Lactobacillus plantarum UALp-05M | Reduced Median Levels of IgA-tTG | Beneficial | Moderate |