Eight-week supplementation of Aronia berry extract promoted the glutathione defence system against acute aerobic exercise-induced oxidative load immediately and 30 min post-exercise in healthy adults: a double-blind, randomised controlled trial.
- 2023-02-20
- Journal of human nutrition and dietetics : the official journal of the British Dietetic Association 36(4)
- Jae-Won Chung
- Ji-Eon Kim
- Yea-Eun Nam
- Woo-Shik Kim
- Insoo Lee
- Sung-Vin Yim
- Oran Kwon
- PubMed: 36727618
- DOI: 10.1111/jhn.13150
Study Design
- Type
- Randomized Controlled Trial (RCT)
- Sample size
- n = 70
- Population
- healthy middle-aged adults with habitually low intakes of fruit and vegetables
- Methods
- double-blind, randomised, controlled clinical trial with two arms (n = 70); 8 weeks of 300 mg Aronia berry extract (ABE) supplementation; acute aerobic exercise (AAE) challenge model (treadmill exercise for 30 min at 60% VO2 max)
- Blinding
- Double-blind
- Duration
- 8 weeks
Background
Food antioxidants have received prompt attention for controlling oxidative stress encountered in daily life. This study aimed to examine the protective effects of Aronia berry extract (ABE) supplementation on acute aerobic exercise (AAE)-induced oxidative stress in healthy subjects.Methods
We assessed a battery of antioxidant defence and oxidative stress parameters at pre-exercise, immediately post-exercise and 30 min post-exercise in healthy middle-aged adults with habitually low intakes of fruit and vegetables in an 8-week, double-blind, randomised, controlled clinical trial with two arms (n = 70). The AAE challenge model, characterised as a treadmill exercise for 30 min at 60% VO2 maximum, was applied to load oxidative stress at the end of the study. Pearson's correlation analysis assessed the association between the changes in antioxidant defence capacities and oxidative stress levels.Results
The time-course-dependent oxidative stress was well observed in the placebo group regarding the glutathione peroxidase (GPx) activity and the reduced glutathione (GSH) availability for antioxidant defence and erythrocyte malondialdehyde, interleukin-6 and lactate levels for oxidative damage. Meanwhile, the ABE supplementation effectively strengthened the glutathione defence system by increasing GSH availability and GPx activity immediately post-exercise and 30 min post-exercise. In addition, the scatter plot and linear regression analysis revealed strong negative correlations of GSH availability with oxidised low-density lipoprotein and plasma malonaldehyde levels.Conclusion
These findings suggest that daily supplementation of 300 mg ABE might help boost GSH levels and an adaptive antioxidant enzyme defence system of erythrocytes in healthy adults with habitually low fruit and vegetable intakes.Research Insights
the ABE supplementation effectively strengthened the glutathione defence system by increasing GSH availability and GPx activity immediately post-exercise and 30 min post-exercise.
- Effect
- Beneficial
- Effect size
- Small
- Dose
- 300 mg/day
the ABE supplementation effectively strengthened the glutathione defence system by increasing GSH availability and GPx activity immediately post-exercise and 30 min post-exercise.
- Effect
- Beneficial
- Effect size
- Small
- Dose
- 300 mg/day
The time-course-dependent oxidative stress was well observed in the placebo group regarding ... erythrocyte malondialdehyde, interleukin-6 and lactate levels.
- Effect
- Neutral
- Effect size
- Small
- Dose
- 300 mg/day
The time-course-dependent oxidative stress was well observed in the placebo group regarding ... erythrocyte malondialdehyde, interleukin-6 and lactate levels.
- Effect
- Neutral
- Effect size
- Small
- Dose
- 300 mg/day
the scatter plot and linear regression analysis revealed strong negative correlations of GSH availability with oxidised low-density lipoprotein and plasma malonaldehyde levels.
- Effect
- Neutral
- Effect size
- Small
- Dose
- 300 mg/day
the scatter plot and linear regression analysis revealed strong negative correlations of GSH availability with oxidised low-density lipoprotein and plasma malonaldehyde levels.
- Effect
- Neutral
- Effect size
- Small
- Dose
- 300 mg/day